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Purified Mouse Anti-FXR2
Purified Mouse Anti-FXR2

Western blot analysis of FXR2 on a PC12 cell lysate (Rat neuroblastoma; ATCC CRL-1721). Lane 1: 1:250, lane 2: 1:500, lane 3: 1000 dilution of the mouse anti-FXR2 antibody.

Purified Mouse Anti-FXR2

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2).

 

Western blot analysis of FXR2 on a PC12 cell lysate (Rat neuroblastoma; ATCC CRL-1721). Lane 1: 1:250, lane 2: 1:500, lane 3: 1000 dilution of the mouse anti-FXR2 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2).

 

製品詳細
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BD Transduction Laboratories™
Fragile X
Rat (QC Testing), Human (Tested in Development)
Mouse IgG2b
Human FXR2 aa. 520-639
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
95 kDa
250 µg/ml
AB_398856
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611330 Rev. 1
抗体の詳細
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55/FXR2

The fragile X syndrome results from amplification of a polymorphic CGG repeat in the 5' untranslated region of the FMR1 gene and is the most common form of inherited mental retardation. Although the lack of expression of FMR1 protein produces the fragile X syndrome, the pathological mechanism is not known. FMR1 is an RNA binding protein that performs a chaperone or transporter function. It interacts with FXR1 and FXR2. These proteins have 60% identity with FMR1 and have similar KH and RNA binding domains. All three proteins are cytosolic, associate with the 60S ribosomal subunit, and form homomers or heteromers with each other. During embryonic development, FMR1, FXR1, and FXR2 are expressed primarily in the nervous system. Small amounts of FMR1, FXR1, and FXR2 shuttle between the cytoplasm and nucleus. FMR1 is found in the nucleoplasm, while FXR2 is localized to the nucleolus. Thus, FMR1, FXR1, and FXR2, either individually or in tandem, function to transport RNAs throughout the cytoplasm and to specific sites in the nucleus. Alterations in the interactions between these proteins and ribosomal components may underlie the pathology that produces fragile X syndrome.

611330 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611330 Rev.1
引用&参考文献
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開発者向け参考資料 (4)

  1. Agulhon C, Blanchet P, Kobetz A. Expression of FMR1, FXR1, and FXR2 genes in human prenatal tissues. J Neuropathol Exp Neurol. 1999; 58(8):867-880. (Biology). 参考文献を見る
  2. Siomi MC, Zhang Y, Siomi H, Dreyfuss G. Specific sequences in the fragile X syndrome protein FMR1 and the FXR proteins mediate their binding to 60S ribosomal subunits and the interactions among them. EMBO J. 1996; 16(7):3825-3832. (Biology). 参考文献を見る
  3. Tamanini F, Bontekoe C, Bakker CE. Different targets for the fragile X-related proteins revealed by their distinct nuclear localizations. Hum Mol Genet. 1999; 8(5):863-869. (Biology). 参考文献を見る
  4. Zhang Y, O'Connor JP, Siomi MC. The fragile X mental retardation syndrome protein interacts with novel homologs FXR1 and FXR2. EMBO J. 1995; 14(21):5358-5366. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす
611330 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.