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Western blot analysis of FKBP65 on a PC-12 cell lysate (Rat neuroblastoma; ATCC CRL-1721) using 0.5 µg/mL of the Mouse Anti-FKBP65 antibody.
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
BD Pharmingen™ Purified Mouse Anti-FKBP65
BD Pharmingen™ Purified Mouse Anti-FKBP65
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
FK506, one of multiple potent compounds that block T cell proliferation, is used for immunosuppressive therapy. Several FK506-binding proteins (FKBPs) have been identified in the search for intracellular targets for FK506. These include FKBP12, FKBP13, FKBP51, and FKBP52. The immunosuppressive effects of FK506 and FKBPs result from the inhibition of the calcineurin phosphatase, a well known component of the signaling cascade leading to IL-2 production. FKBP65 is another member of the FKBP protein family and contains the characteristic peptidylprolyl cis-trans-isomerase activity. FKBP65 has been reported to be expressed in the lung, testis, brain, heart, and spleen. Although it has a predicted molecular weight of 65 kDa, FKBP65 has been observed to migrate ranging from 68-72 kDa in SDS electrophoresis gels, perhaps, due to glycosylation and/or phosphorylation.
Development References (2)
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Coss MC, Winterstein D, Sowder RC 2nd, Simek SL. Molecular cloning, DNA sequence analysis, and biochemical characterization of a novel 65-kDa FK506-binding protein (FKBP65). J Biol Chem. 1995; 270(49):29336-29341. (Biology). View Reference
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Patterson CE, Schaub T, Coleman EJ, Davis EC. Developmental regulation of FKBP65. An ER-localized extracellular matrix binding-protein. Mol Biol Cell. 2000; 11(11):3925-3935. (Biology: Immunofluorescence, Immunohistochemistry, Western blot). View Reference
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