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Purified Mouse Anti-DSIF
Purified Mouse Anti-DSIF

Western blot analysis of DSIF on a HeLa lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- DSIF antibody.

Purified Mouse Anti-DSIF

Immunoflourescence staining of human endothelial cells.

Western blot analysis of DSIF on a HeLa lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- DSIF antibody.

Immunoflourescence staining of human endothelial cells.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human DSIF aa. 866-985
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
160 kDa
250 µg/ml
AB_398420
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611106 Rev. 1
抗体の詳細
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17/DSIF

In living systems, the relative amounts of any protein are controlled at many levels. For example, amounts are affected by protein degradation, regulation of the translational rates of polypeptide synthesis (translational regulation), and control of the rates of mRNA synthesis (transcriptional regulation). Transcriptional regulation involves modulation of the rate-limiting enzyme RNA polymerase. DSIF (DRB sensitivity-inducing factor) is a heterodimeric transcription elongation protein. It is composed of a large subunit of 160 kDa and a small subunit of 14 kDa. These large and the small subunits are homologs of the yeast gene products Stp5 and Stp4, respectively. Spt4 and 5 are transcription factors which are critically important for the activity of RNA polymerase. In conjunction with DRB, DSIF attenuates RNA polymerase II elongation steps. However, in limiting amounts of ribonucleotides, DSIF, by itself, stimulates the elongation rate of RNA polymerase II. Thus, the identification of a human regulator for transcriptional elongation will greatly enhance our understanding of this critical step in mammalian gene expression.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611106 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611106 Rev.1
引用&参考文献
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開発者向け参考資料 (1)

  1. Kim YK, Bourgeois CF, Isel C, Churcher MJ, Karn J. Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation. Mol Cell Biol. 2002; 22(13):4622-4637. (Biology: Western blot). 参考文献を見る
611106 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.