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Purified Mouse Anti- CUL-2
Purified Mouse Anti- CUL-2

Western blot analysis of CUL-2 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- CUL-2 antibody.

Purified Mouse Anti- CUL-2

Immunofluorescence staining of human endothelial cells.

Western blot analysis of CUL-2 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- CUL-2 antibody.

Immunofluorescence staining of human endothelial cells.

製品詳細
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BD Transduction Laboratories™
Cullin-2
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human CUL-2 aa. 18-182
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
76 kDa
250 µg/ml
AB_398099
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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抗体の詳細
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4/CUL-2

The cullins are a family of proteins that are integral to cell cycle regulation.  Members of this family include human Cul-2, C.elegans Ce-Cul-1, and S. cerevisiae Cdc53.  Proper control of cell cycle progression is essential for the prevention of tumorigenesis.  Mutant forms of both Ce-Cul-1 and Cdc53 have been implicated in the oncogenic process.  Abnormal nuclear localization of Cul-2 is seen in a rare hereditary condition known as VHL (von Hippel-Lindau) syndrome.  Patients with VHL syndrome possess a mutant VHL gene and develop cancers such as retinal anginomas, CNS hemangioblastomas, and renal cell carcinomas.  In normal cells, the VHL protein competes with subunit A of elongin, a transcription elongation factor, for binding to subunits B and C.  The formation of the VHL-B-C complex inhibits elongation.  Additional data indicates that this complex associates with CUL-2 and promotes CUL-2 nuclear translocation.  In cells with mutated VHL, transcriptional elongation is not efficiently curtailed and CUL-2 is not properly localized in the nucleus.  Therefore, CUL-2 mediates VHL function, suggesting CUL-2 may be a candidate human tumor suppressor.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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引用&参考文献
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開発者向け参考資料 (2)

  1. Pause A, Lee S, Worrell RA, et al. The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc Natl Acad Sci U S A. 1997; 94(6):2156-2161. (Biology). 参考文献を見る
  2. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology: Immunofluorescence). 参考文献を見る
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.