Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified NA/LE Rat Anti-Mouse IL-10
      製品詳細
      Down Arrow Up Arrow


      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG1, κ
      Mouse IL-10
      ELISA (Routinely Tested), Neutralization (Tested During Development)
      1.0 mg/ml
      15978
      AB_395381
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation and Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      推奨アッセイ手順

      ELISA:  Purified Rat Anti-Mouse IL-10 antibody (Clone JES5-2A5, Cat. No. 551215) has been reported to be useful as a capture antibody in sandwich ELISA for mouse IL-10.  Purified JES5-2A5 antibody can be paired with the Biotin Rat Anti-Mouse IL-10 antibody (Clone SXC-1, Cat. No. 554423) or Biotin Rat Anti-Mouse IL-10 antibody (Clone JES5-16E3, Cat. No. 554465) and recombinant mouse IL-10 (Cat. No. 550070) as the standard.  Purified JES5-2A5 antibody should be titrated (suggested 2-6 µg/mL) to determine the optimal concentration for ELISA capture. An appropriate range of concentrations of the mouse IL-10 standard for obtaining a linear standard curve is 2 ng/mL to 15 pg/mL mouse IL-10.

      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow
      554421 Rev. 4
      抗体の詳細
      Down Arrow Up Arrow
      JES5-2A5

      The JES5-2A5 antibody reacts with mouse IL-10. The immunogen used to generate the JES5-2A5 hybridoma was E. coli-expressed mouse IL-10. This is a neutralizing antibody.

      554421 Rev. 4
      フォーマットの詳細
      Down Arrow Up Arrow
      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554421 Rev.4
      引用＆参考文献
      Down Arrow Up Arrow

      Development References (5)

      1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
      2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization). View Reference
      3. Mo XY, Sarawar SR, Doherty PC. Induction of cytokines in mice with parainfluenza pneumonia. J Virol. 1995; 69(2):1288-1291. (Clone-specific: ELISA). View Reference
      4. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA). View Reference
      5. Sarawar SR, Sangster M, Coffman RL, Doherty PC. Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype. J Immunol. 1994; 153(3):1246-1253. (Clone-specific: ELISA). View Reference
      すべて表示する (5) 表示項目を減らす
      554421 Rev. 4

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback