BD Pharmingen™ Purified Rat Anti-Mouse IL-9

ELISA Capture: Purified D8402E8 antibody (Cat. No. 551218) is useful as a capture antibody for a sandwich ELISA for measuring mouse IL-9 protein levels.  Purified D8402E8 antibody can be paired with the biotinylated D9302C12 antibody as the detecting antibody (Cat. No. 554473), with recombinant mouse IL-9 as the standard. Purified D8402E8 antibody should be titrated 2.0 - 6.0 µg/ml to determine optimal concentration for ELISA capture. For maximal sensitivity, Pharmingen recommends an overnight incubation (4°C) of samples/standards with the coated capture antibody. For specific methodology, please visit our web site, www.bdbiosciences.com , and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

        This ELISA pair shows no cross-reactivity with any of the cytokines tested ( e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL- 7, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGFAA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).

        For neutralization of mouse IL-9 bioactivity, we recommend an alternate clone, the D9302C12 antibody in the NA/LE™ format (Cat. No. 554472).

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.