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R718 Mouse Anti-Human TCRαβ
R718 Mouse Anti-Human TCRαβ

Multicolor flow cytometric analysis of TCRαβ expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD19 antibody (Cat. No. 659477), FITC Mouse Anti-Human CD3 antibody (Cat. No. 555916/555332/561807/561806) and either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat No. 566928; Left Plots) or BD Horizon™ R718 Mouse Anti-Human TCRαβ antibody (Cat. No. 567676/567677; Right Plots). Erythrocytes were lysed with BD FACSLyse™ Lysing Buffer (Cat. No. 349202). Flow cytometric analysis was performed using a LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software.

Upper Plots:  Bivariate pseudocolor density plots showing the correlated expression of CD19 versus TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD3 versus TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

Multicolor flow cytometric analysis of TCRαβ expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD19 antibody (Cat. No. 659477), FITC Mouse Anti-Human CD3 antibody (Cat. No. 555916/555332/561807/561806) and either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat No. 566928; Left Plots) or BD Horizon™ R718 Mouse Anti-Human TCRαβ antibody (Cat. No. 567676/567677; Right Plots). Erythrocytes were lysed with BD FACSLyse™ Lysing Buffer (Cat. No. 349202). Flow cytometric analysis was performed using a LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software.

Upper Plots:  Bivariate pseudocolor density plots showing the correlated expression of CD19 versus TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD3 versus TCRαβ (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.

製品詳細
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BD Horizon™
alpha-beta T cell receptor, TCRAB, TRA@/TRB@; IP26A
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human T Cell Clone
Flow cytometry (Routinely Tested)
5 µl
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


調製と保管

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

製品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
567676 Rev. 1
抗体の詳細
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IP26

The IP26 monoclonal antibody specifically recognizes a monomorphic determinant on the αβ T-cell receptor (TCRαβ). The TCRαβ is a disulfide-linked 80 kDa heterodimer consisting of a 44 kDa α chain and a 37 kDa β chain. The TCRαβ is normally expressed on 50-70% of thymocytes and on a large fraction of mature T cells including greater than 95% of peripheral blood CD3+ T cells. The TCRαβ serves as a receptor for peptide antigens bound to MHC molecules. The IP26 antibody is mitogenic and useful for the flow cytometric analysis of TCRαβ+ T-cell subsets.

The antibody was conjugated to BD Horizon™ Red 718, which has been developed exclusively by for BD Biosciences as a better alternative to Alexa Fluor™ 700. BD Horizon™ Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor™ 700.

567676 Rev. 1
フォーマットの詳細
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
567676 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Agrawal SG, Marquet J, Plumas J, et al. Multiple co-stimulatory signals are required for triggering proliferation of T cells from human secondary lymphoid tissue. Int Immunol. 2001; 13(4):441-450. (Clone-specific: Activation, Functional assay, Stimulation). 参考文献を見る
  2. Nikolova M, Marie-Cardine A, Boumsell L, Bensussan A. BY55/CD160 acts as a co-receptor in TCR signal transduction of a human circulating cytotoxic effector T lymphocyte subset lacking CD28 expression. Int Immunol. 2002; 14(5):445-451. (Clone-specific: Flow cytometry). 参考文献を見る
  3. Oettgen HC, Kappler J, Tax WJ, Terhorst C. Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J Biol Chem. 1984; 259(19):12039-12048. (Biology). 参考文献を見る
  4. Ortonne N, Huet D, Gaudez C, et al. Significance of circulating T-cell clones in Sezary syndrome. Blood. 2006; 107(10):4030-4038. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). 参考文献を見る
  5. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Clone-specific: Flow cytometry). 参考文献を見る
すべて表示する (5) 表示項目を減らす
567676 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.