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R718 Mouse Anti-Human HLA-A
R718 Mouse Anti-Human HLA-A
Multiparameter flow cytometric analysis of HLA-A expression on human peripheral blood leucocyte populations. Human whole blood was stained with BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-Human HLA-A antibody (Cat. No. 568030/568031; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of HLA-A (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Multiparameter flow cytometric analysis of HLA-A expression on human peripheral blood leucocyte populations. Human whole blood was stained with BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-Human HLA-A antibody (Cat. No. 568030/568031; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of HLA-A (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
製品詳細
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BD Horizon™
HLA-A; HLAA; MHC class I antigen HLA-A heavy chain
Human (QC Testing)
Mouse IgG1, κ
Human Peripheral Blood Lymphocytes
Flow cytometry (Routinely Tested)
5 µl
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

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BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
  8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
568031 Rev. 1
抗体の詳細
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1082C5

The 1082C5 monoclonal antibody (also known as clone 108-2C5) recognizes a shared antigenic determinant present on the extracellular region of Human Leukocyte Antigen-A (HLA-A) heavy chains encoded by a limited number of different HLA-A alleles (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -A33). HLA-A antigens belong to the major histocompatibility complex (MHC) of class I antigens along with HLA-B and HLA-C antigens. HLA class I molecules are heterodimers comprised of an ~40-45 kDa, highly polymorphic transmembrane α heavy chain that is a type I glycoprotein which is noncovalently-associated with an invariant β2-microglobulin (β2m) light chain. The N-terminal extracellular region of the HLA class I heavy chain is comprised of three domains (α1, α2, and α3). The α1 and α2 domains form a closed antigen-binding groove that accommodates 8-10 aa-peptide antigens. β2m non-covalently associates with the α3 heavy chain domain and promotes HLA class I stability. The intralocus HLA-A determinant recognized by the 108-2C5 antibody reportedly involves amino acids residues 76-80 of the HLA-A α1 domain. HLA-A antigens are normally expressed on all nucleated cells. These molecules play central roles in the MHC class I-restricted presentation and cross-presentation of antigens and the regulation of NK and T cell-mediated cytotoxicity that are involved in immune responses to pathogens and tumors as well as tissue allotransplantation.

568031 Rev. 1
フォーマットの詳細
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
568031 Rev.1
引用&参考文献
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View product citations for antibody "568031" on CiteAb

Development References (6)

  1. Bardi MS, Jarduli LR, Jorge AJ, et al. HLA-A, B and DRB1 allele and haplotype frequencies in volunteer bone marrow donors from the north of Parana State. Rev Bras Hematol Hemoter. 2012; 34(1):25-30. (Biology). View Reference
  2. Guerreiro-Cacais AO, Uzunel M, Levitskaya J, Levitsky V. Inhibition of heavy chain and beta2-microglobulin synthesis as a mechanism of major histocompatibility complex class I downregulation during Epstein-Barr virus replication.. J Virol. 2007; 81(3):1390-400. (Clone-specific: Flow cytometry). View Reference
  3. Hühn MH, Hultcrantz M, Lind K, Ljunggren HG, Malmberg KJ, Flodström-Tullberg M. IFN-gamma production dominates the early human natural killer cell response to Coxsackievirus infection.. Cell Microbiol. 2008; 10(2):426-36. (Clone-specific: Flow cytometry). View Reference
  4. Llano M, Gumá M, Ortega M, Angulo A, López-Botet M. Differential effects of US2, US6 and US11 human cytomegalovirus proteins on HLA class Ia and HLA-E expression: impact on target susceptibility to NK cell subsets.. Eur J Immunol. 2003; 33(10):2744-54. (Clone-specific: Flow cytometry). View Reference
  5. Lozano F, Santos-Aguado J, Borche L, et al. Identification of the amino acid residues defining an intralocus determinant in the alpha 1 domain of HLA-A molecules.. Immunogenetics. 1989; 30(1):50-3. (Clone-specific: Blocking, Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
  6. Ryschich E, Cebotari O, Fabian OV, et al. Loss of heterozygosity in the HLA class I region in human pancreatic cancer.. Tissue Antigens. 2004; 64(6):696-702. (Clone-specific: Immunohistochemistry). View Reference
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568031 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.