Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu
Website Feedback
Alert Icon
Purified NA/LE Rat Anti-Mouse LPAM-1
製品詳細
Down Arrow Up Arrow


BD Pharmingen™
Itga4Itgb7; α4β7 Integrin; alpha 4 beta 7 integrin; CD49dβ7
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
AKR/Cum mouse T lymphoma line TK1
Flow cytometry (Routinely Tested), Blocking, Induction (Reported), Immunohistochemistry (Not Recommended)
1.0 mg/ml
AB_395065
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
詳細を表示 blue down arrow 表示項目を減らす blue up arrow

推奨アッセイ手順

Flow cytometry: Because expression of LPAM-1 on peripheral lymphocytes is at very low density, we recommend amplification of the signal through the use of a biotinylated second-step antibody plus a "bright" third-step reagent, such as Streptavidin-PE (Cat. no. 554061), for detection of the antigen by flow cytometry. Other reported applications include in vitro blocking of adhesion, induction of cellular aggregation, and in vivo blocking of lymphocyte homing to mucosal sites.

詳細を表示 blue down arrow 表示項目を減らす blue up arrow

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
詳細を表示 blue down arrow 表示項目を減らす blue up arrow
553809 Rev. 11
抗体の詳細
Down Arrow Up Arrow
DATK32

The DATK32 monoclonal antibody specifically binds to an epitope specific for the α4β7 integrin heterodimer.  α4β7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells.  It interacts with several ligands, including VCAM-1 (CD106), fibronectin, and MAdCAM-1.  DATK32  antibody induces α4β7-dependent lymphocyte aggregation, but it inhibits other α4β7- mediated lymphocyte adhesion events, including binding to fibronectin, MAdCAM-1, and VCAM-1 (CD106).

553809 Rev. 11
フォーマットの詳細
Down Arrow Up Arrow
NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is =0.01 EU/µg (=0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
553809 Rev.11
引用＆参考文献
Down Arrow Up Arrow
View product citations for antibody "553809" on CiteAb

Development References (5)

  1. Andrew DP, Berlin C, Honda S, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994; 153(9):3847-3861. (Immunogen: Blocking, Induction). View Reference
  2. Andrew DP, Rott LS, Kilshaw PJ, Butcher EC. Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes. Eur J Immunol. 1996; 26(4):897-905. (Clone-specific: Blocking). View Reference
  3. Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. (Clone-specific: Blocking). View Reference
  4. Hamann A, Andrew DP, Jablonski-Westrich D, Holzmann B, Butcher EC. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol. 1994; 152(7):3282-3293. (Clone-specific: Blocking). View Reference
  5. Kilshaw PJ, Murant SJ. Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system. Eur J Immunol. 1991; 21(10):2591-2597. (Biology). View Reference
すべて表示する (5) 表示項目を減らす
553809 Rev. 11

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.