BD Pharmingen™ Purified Mouse anti-Syk (pY348)
クローン I120-722 (RUO)
Lysate from control (left panel) and pervanadate-treated (right panel) Ramos cells (Burkitt's lymphoma) were probed with mAb I120-722 at concentrations of 0.25 (lanes 1), 0.125 (lanes 2), and 0.0625 (lanes 3) µg/ml. Syk (pY348) is identified as a strong band of 72 kDa in the pervanadate-treated Ramos cells.
Syk (pY348) staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadate solution for 2 hours (top row) or unstimulated (bottom row), was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with Purified Mouse anti-Syk (pY348) monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X.
Lysate from control (left panel) and pervanadate-treated (right panel) Ramos cells (Burkitt's lymphoma) were probed with mAb I120-722 at concentrations of 0.25 (lanes 1), 0.125 (lanes 2), and 0.0625 (lanes 3) µg/ml. Syk (pY348) is identified as a strong band of 72 kDa in the pervanadate-treated Ramos cells.
Syk (pY348) staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadate solution for 2 hours (top row) or unstimulated (bottom row), was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with Purified Mouse anti-Syk (pY348) monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Syk is a non-receptor protein-tyrosine kinase that is closely related to ZAP70 and plays crucial roles in the development and receptor-mediated signaling of most leukocytes and in vascular integrity. Syk is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. Mature (CD4 or CD8 single-positive) thymocytes and peripheral αβ TCR-bearing T lymphocytes have very low or undetectable levels of Syk. Syk contributes to the signal transduction process by binding to ITAMs (Immunoreceptor Tyrosine-based Activation Motifs) of immune receptors, including Igα and Igβ (CD79a and b), TCRζ, CD3ε, and FcRγ. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in the interdomain, between the SH2 and Kinase domains of Syk, to undergo auto-phosphorylation and phosphorylation by Lyn. The tyrosine 348 phosphorylation site (pY348) in human Syk is orthologous to tyrosine 342 in mouse and rat Syk and tyrosine 315 in human ZAP70. This phosphorylated site can act as a binding site for other signaling molecules, such as PLCγ, Vav, and Fgr.
The I120-772 antibody is specific for human Syk (pY348) and does not cross-react with phosphorylated Zap70. The orthologous phosphorylation site in mouse and rat Syk is Y342.
Development References (7)
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Abtahian F, Guerriero A, Sebzda E, et al. . Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk. Science. 2003; 299:247-251. (Biology).
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Coopman PJP, Do MTH, Barth M, et al. The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells. Nature. 2000; 406:742-747. (Biology).
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Hong JJ, Yankee TM, Harrison ML, Geahlen RL. Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. J Biol Chem. 2002; 277:31703-31714. (Biology).
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Latour S, Veillette A. Proximal protein tyrosine kinases in immunoreceptor signaling. Curr Opin Immunol. 2001; 13:299-306. (Biology).
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Turner M, Schweighoffer E, Colucci F, Di Santo JP, Tybulewicz VL. Tyrosine kinase SYK; essential functions for immunoreceptor signaling. Immunol Today. 2000; 21:148-154. (Biology). View Reference
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Vines CM, Potter JW, Xu Y, et al. Inhibition of β2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr. Immunity. 2001; 15:507-519. (Biology).
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Zhang J, Benestein E, Siraganian RP. Phosphorylation of Tyr342 in the linker region of Syk is critical for FceRI signaling in mast cells. Mol Cell Biol. 2002; 22:8144-8154. (Biology).
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