Preparation and Storage
推奨アッセイ手順
Immunohistochemistry: The 1C7 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were rat spleen and thymus. The antibody stains macrophages, monocytes, and dendritic cells. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, the 1C7 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-mouse Ig (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). For more protocol information please visit http://www.bdbiosciences.com/resources/cellbiology/index.jsp This product is not recommended to be used on formalin fixed paraffin tissue sections.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The 1C7 monoclonal antibody reacts with an antigen found on almost all cells of the mononuclear phagocyte system. Its cellular distribution is reported to be identical to that of CD68 (ED1 Antigen), which has been detected on tissue macrophages, dendritic cells, osteoclasts, resident peritoneal macrophages, alveolar macrophages, and peripheral blood monocytes, but not on granulocytes or lymphocytes. The distribution of ED1+ myeloid cells is similar to that of RT1B (rat I-A equivalent), and all ED1+ cells have acid phosphatase activity.
Development References (3)
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Dijkstra CD, Döpp EA, Joling P, Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985; 54(3):589-599. (Biology). View Reference
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de Jong, M. W. A. The 1C7 antibody reacts with an antigen found on almost all cells of the mononuclear phagocyte system. .
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van Goor H, Harms G, Gerrits PO, Kroese FG, Poppema S, Grond J. Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue. J Histochem Cytochem. 1988; 36(1):115-120. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
