Skip to main content Skip to navigation
Purified Mouse Anti-Rat CD4
Purified Mouse Anti-Rat CD4

Immunohistochemical staining of CD4+ T lymphocytes. Frozen sections of normal rat spleen were stained with the anti-rat CD4 clone OX-35 antibody. CD4+ T lymphocytes in the periarteriolar sheath are identified by the brown staining.

Immunohistochemical staining of CD4+ T lymphocytes. Frozen sections of normal rat spleen were stained with the anti-rat CD4 clone OX-35 antibody. CD4+ T lymphocytes in the periarteriolar sheath are identified by the brown staining.

製品詳細
Down Arrow Up Arrow


BD Pharmingen™
Cd4; CD4 antigen; p55; W3/25 antigen; T-cell surface glycoprotein CD4
Rat (QC Testing)
Mouse BALB/c IgG2a, κ
Rat T-cell blasts
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
62.5 µg/ml
AB_393591
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


推奨アッセイ手順

Immunohistochemistry: Clone OX-35 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections of rat spleen and thymus. IHC of formalin-fixed paraffin embedded sections is not recommended.  This antibody has been reported to stain the CD4 subset of T lymphocytes. The isotype control recommended for use with this antibody is purified mouse IgG2a (Cat. No. 550339). For optimal indirect immunohistochemical staining, the OX-35 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotin conjugated rat anti-mouse IgG2a (Cat. No. 550332) as the secondary antibody and streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550296 Rev. 2
抗体の詳細
Down Arrow Up Arrow
OX-35

The OX-35 clone recognizes the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase Lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones.

550296 Rev. 2
フォーマットの詳細
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550296 Rev.2
引用&参考文献
Down Arrow Up Arrow

開発者向け参考資料 (7)

  1. Bañuls MP, Alvarez A, Ferrero I, Zapata A, Ardavin C. Cell-surface marker analysis of rat thymic dendritic cells. Immunology. 1993; 79(2):298-304. (Biology). 参考文献を見る
  2. Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599. (Biology). 参考文献を見る
  3. Janeway CA Jr. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992; 10:645-674. (Biology). 参考文献を見る
  4. Jefferies WA, Green JR, Williams AF. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985; 162(1):117-127. (Immunogen: Flow cytometry, Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Inhibition). 参考文献を見る
  5. Liu L, Zhang M, Jenkins C, MacPherson GG. Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression. J Immunol. 1998; 161(3):1146-1155. (Biology). 参考文献を見る
  6. McElwee KJ, Spiers EM, Oliver RF. Partial restoration of hair growth in the DEBR model for Alopecia areata after in vivo depletion of CD4+ T cells. Br J Dermatol. 1999; 140(3):432-437. (Clone-specific: Depletion). 参考文献を見る
  7. Morrison WJ, Kennedy NJ, Offner H, Vandenbark AA. Effects of anti-CD4 antibody: enhancement of lymph node PPD-memory T cell response. Cell Immunol. 1995; 163(1):106-112. (Clone-specific: Depletion). 参考文献を見る
すべて表示する (7) 表示項目を減らす
550296 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.