Skip to main content Skip to navigation
Purified Mouse Anti-Human MUC1 (CD227)
Purified Mouse Anti-Human MUC1 (CD227)

Immunohistochemistry of MUC1 positive cells. Formalin-fixed paraffin embedded sections of human breast cancer were reacted with Purified Mouse Anti-Human MUC1 (CD227) antibody (Cat. No. 550486). Three-step staining and visualization was carried out with Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550337), Streptavidin HRP (Cat. No. 550946), and DAB Substrate Kit (Cat. No. 550880). Tumor cells expressing MUC1 can be identified by the intense brown labeling of their cell membranes. Magnification 20X.

Immunohistochemistry of MUC1 positive cells. Formalin-fixed paraffin embedded sections of human breast cancer were reacted with Purified Mouse Anti-Human MUC1 (CD227) antibody (Cat. No. 550486). Three-step staining and visualization was carried out with Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550337), Streptavidin HRP (Cat. No. 550946), and DAB Substrate Kit (Cat. No. 550880). Tumor cells expressing MUC1 can be identified by the intense brown labeling of their cell membranes. Magnification 20X.

製品詳細
Down Arrow Up Arrow


BD Pharmingen™
MUC1; CA 15-3; DF3; EMA; Episialin; H23; H23AG; KL-6; MAM6; PEM; PEMT; PUM
Human (QC Testing)
Mouse IgG1, κ
Human Milk Fat Globule Membranes
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
500 µg/ml
4582
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


推奨アッセイ手順

Immunohistochemistry: The HMPV clone reactive against human MUC1 is recommended to test for immunohistochemical staining of formalin-fixed paraffin and acetone-fixed frozen sections. For paraffin sections, microwave pretreatment with BD Retrievagen A (pH 6.5) (Cat. No. 550524) is required. Tissue tested was human breast cancer tissue. The antibody stains normal and cancerous cells with a high carbohydrate content. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, HMPV antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the Anti-Mouse Ig HRP detection kit (Cat. No. 551011) that contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate can be used for staining. A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources.

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  6. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550486 Rev. 3
抗体の詳細
Down Arrow Up Arrow
HMPV

The HMPV monoclonal antibody specifically binds to CD227 which is also known as Mucin-1 (MUC1).  A major form of CD227 is expressed as a type I transmembrane glycoprotein. CD227 belongs to the epithelial mucin family whose members are heavily O-glycosylated and characterized by high molecular weight, and an amino acid composition rich in serine, threonine, proline, and glycine. CD227 is variably expressed on the surfaces of normal and malignant glandular and ductal epithelial cells, and some hematopoietic cell lineages including subsets of T cells, B cells, monocytes and dendritic cells. Soluble forms of CD227 may arise by shedding from the cell surface or by secretion of forms derived from alternative RNA splicing. The HMPV antibody binds to the core peptide of the MUC1 protein. The core protein contains a domain of 20 amino-acid tandem repeats which function as multiple epitopes for this monoclonal antibody. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in immunostaining intensities between cells from normal and malignant tissues. CD227 plays roles in the provision of protective barrier function, the regulation of cellular adhesion, and the transduction of multiple signal pathways.

550486 Rev. 3
フォーマットの詳細
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550486 Rev.3
引用&参考文献
Down Arrow Up Arrow

開発者向け参考資料 (3)

  1. Devine PL, Birrell GW, Whitehead RH, Harada H, Xing PX, McKenzie IF. Expression of MUC1 and MUC2 mucins by human tumor cell lines. Tumour Biol. 1992; 13(5):268-277. (Biology). 参考文献を見る
  2. Xing PX, Prenzoska J, Layton GT, Devine PL, McKenzie IF. Second-generation monoclonal antibodies to intestinal MUC2 peptide reactive with colon cancer. J Natl Cancer Inst. 1992; 84(9):699-703. (Biology). 参考文献を見る
  3. Xing PX, Prenzoska J, Quelch K, McKenzie IF. Second generation anti-MUC1 peptide monoclonal antibodies. Cancer Res. 1992; 52(8):2310-2317. (Biology). 参考文献を見る
550486 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.