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Purified Mouse Anti-Human IL-8
Purified Mouse Anti-Human IL-8
Immunocytochemistry analysis of IL-8 expression on human peripheral blood mononuclear cells. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37°C with LPS (Sigma No. L-8274, 1 µg / ml) in the presence of GolgiStop™ (Cat. No. 554724). The activated cells were harvested and the level of IL-8 producing cells was detected by immunocytochemistry using a three-step staining procedure that employs a Biotin Goat anti-mouse IgG secondary antibody (Cat. No. 550337) and Streptavdin-horseradish peroxidase (Cat. No. 550946) (Nomarski optics, original magnification 400X).
Immunocytochemistry analysis of IL-8 expression on human peripheral blood mononuclear cells. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37°C with LPS (Sigma No. L-8274, 1 µg / ml) in the presence of GolgiStop™ (Cat. No. 554724). The activated cells were harvested and the level of IL-8 producing cells was detected by immunocytochemistry using a three-step staining procedure that employs a Biotin Goat anti-mouse IgG secondary antibody (Cat. No. 550337) and Streptavdin-horseradish peroxidase (Cat. No. 550946) (Nomarski optics, original magnification 400X).
製品詳細
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BD Pharmingen™
IL8; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1; emoctakin
Human (QC Testing)
Mouse IgG2b
Recombinant Human IL-8
Intracellular staining (flow cytometry) (Routinely Tested)
0.5 mg/ml
AB_393672
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推奨アッセイ手順

Immunocytochemistry: The purified format of the G265-8 antibody (Cat. No. 550419) can be used to identify and enumerate human IL-8 producing cells by immunocytochemistry. For optimal indirect immunocytochemical staining, the G265-8 antibody should be titrated (≤ 1 µg) and visualized via a three step staining procedure in combination with Biotin Goat Anti-Mouse IgG and  Streptavidin-horseradish peroxidase (HRP). For a detailed description of the immunocytochemical procedure, please see protocol below.

CYTOKINE IMMUNOCYTOCHEMISTRY PROTOCOL

REAGENTS REQUIRED

1. Fixation Buffer: 5% formalin (10% formalin, CMS, Cat. No. 245-684) is dissolved in phosphate buffered-saline (PBS) (Bacto FA Buffer, Difco Laboratories, Cat. No. 2314-15-0), or BD Pharmingen™ ICC Fixation Buffer (BD Cat. No. 550010)

2. Endogenous Peroxidase Blocking Buffer: DAKO Peroxidase Blocking Reagent (DAKO, Cat. No. S2001).

3. Endogenous Biotin Blocking Buffer: Biotin/Avidin Blocking Kit (Vector Laboratories, Cat. No. SP-2001).

4. Antibody dilution buffer:  BD™ Pharmingen Antibody Diluent for IHC (Cat. No. 559148) supplemented with saponin.

5. Microscopic slides: Adhesion Slides (Erie Scientific Company, Cat. No. ER-202B-AD) or for cytospins, Colorfrost/Plus slides (Fisher, Cat. No. 12-550-17).

6. Biotin Goat anti-Mouse IgG (Cat. No. 550337) or the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011).

7. Detection system: BD Pharmingen™ Streptavidin-horseradish peroxidase (HRP), (Cat. No. 550946), or Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011).

8. Mounting medium for short-term storage: Aqua-mount® (Lerner Laboratories, Cat. No. 13800).

9. DAB Substrate Kit (contains 3-3 -Diaminobenzidine tetra hydrochloride), (BD Cat. No. 550880), or Anti-Mouse Ig HRP Detection Kit.

PROCEDURE FOR IMMUNOCYTOCHEMICAL STAINING OF SINGLE-CELL PREPARATIONS

This procedure describes the immunoenzymatic technique of staining cytokine within individual cells that are immobilized on microscopic slides via adherence (adherent slides) or centrifugation (cytospins).

ADHESION SLIDES

1. Harvest cells and wash them twice in PBS using centrifugation (400 x g for 5 min) to remove residual protein.

2. Adjust the cell concentration at 4-5 x 10e6 cells/ml in PBS.

3. Place 20 µl of the cell suspension in each well of the adhesion slides and let them adhere at room temperature (RT) for 20 min. Please note that the slides should be washed in PBS at RT for 5 min before transferring the cells.

4. Fix cells on slides using fixation buffer for 15 min at RT.

5. Wash slides 2X in PBS with 5 min incubations.

6. Block slides with PBS supplemented with 1% (w/v) BSA (Sigma, Cat. No. A43-78) for 30 min at RT or 10 min at 37°C.

7. Wash slides 2X in PBS and proceed with staining or air dry them and store them at -80°C for future use.

8. Incubate slides with 20 µl of 1% goat serum and PBS with 0.1% (w/v) saponin for 30 min at RT.

9. Wash slides 2X with PBS with 5 min incubations.

10. Block endogenous peroxidase activity with Endogenous Peroxidase Blocking Buffer (20 µl/well) for 10 min at RT.

11. Wash 2X in PBS with 5 min incubations.

12. Incubate each well with Avidin (20 µl/well) for 15 min.

13. Wash 2X in PBS with 5 min incubations.

14. Incubate each well with Biotin (20 µl/well) for 15 min.

15. Wash 2X in PBS with 5 min incubations.

16. Incubate each well for 1 hr at RT with 20 µl of purified cytokine-specific antibody or appropriate immunoglobulin isotype control diluted in Antibody Diluent for IHC supplemented with saponin.

17. Wash slides 2X in PBS with 5 min incubations.

18. Incubate each well with 20 µl of a biotinylated secondary antibody diluted in Antibody Diluent for IHC for 30 min at RT.

19. Wash 2X in PBS with 5 min incubations.

20. Apply 20 µl of Streptavidin-HRP (BD Cat. No. 550946) to each well on slides and incubate for 30 min at RT.

21. Wash slides 2X with PBS with 5 minutes incubations.

22. Incubate with DAB Substrate as directed, (BD Cat. No. 550880) for less than 5 min at RT.

23. Stop the development of the color reaction by washing with PBS.

24. The slides are subsequently mounted in short-term storage mounting medium.

CYTOSPINS

1. Assemble the Cytospin's sample chamber (e.g. Cytospin 3, Shandon, UK or comparable centrifuge), filter card, slide and cytospin racks according to manufacturer's specifications.

2. Load 40 µl of approximately 1 x 10e6 cells to each sample chamber.

3. Spin slides at 600 rpm for 2 min.

4. Take slides out of the cytospin rack and place them on a staining rack.

5. For fixation and staining please follow the steps 4 through 24 specified above for staining cells on adhesion slides.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550419 Rev. 2
抗体の詳細
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G265-8

The G265-8 monoclonal antibody specifically binds to both the 72 and 77 amino acid isoforms of human Interleukin-8 (IL-8). IL-8 is secreted as an 8-9 kDa, non-glycosylated proinflammatory chemokine protein also known as chemokine (C-X-C motif) ligand 8 (CXCL8). IL-8 is synthesized as a 99 amino acid precursor that is proteolytically processed into several isoforms. The 72 amino acid isoform is produced by monocytes, macrophages, granulocytes, epithelial cells, and fibroblasts in response to pro-inflammatory stimuli including cytokines and microbial agents. It is also expressed by endothelial cells, fibroblasts, keratinocytes, lymphocytes, and a variety of tumor cells. In response to IL-4, IL-10 and TGFβ, the cellular production of IL-8 is inhibited. IL-8 is crucial for the activation and recruitment of neutrophils to inflammatory sites. IL-8 is also a chemoattractant for basophils and T-lymphocytes. IL-8 possesses angiogenic activity and can be associated with tumor angiogenesis and metastasis. The 77 amino acid IL-8 isoform is primarily produced by endothelial cells. This larger isoform is reportedly a less potent neutrophil activator than the 72 amino acid isoform. IL-8 binds to and signals through two G-protein-coupled receptors, IL-8RA (CXCR1/CD181) and IL-8RB (CXCR2/CD182).

550419 Rev. 2
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550419 Rev.2
引用&参考文献
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Development References (3)

  1. Hsu SM, Raine L, Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol. 1981; 75(5):734-738. (Methodology). View Reference
  2. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-580. (Methodology). View Reference
  3. Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Biology). View Reference
550419 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.