Purified Hamster Anti-Rat CD49b

This is a suggested alternate SKU for 559987. Contact us for additional support.

BD Pharmingen™ Purified Hamster Anti-Rat CD49b

Name: Purified Hamster Anti-Rat CD49b
Brand: Purified Hamster Anti-Rat CD49b
SKU: 554998

クローン Ha1/29 (RUO)

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製品詳細

Brand: BD Pharmingen™

Alternative Name: Itga2; Integrin α2 chain; Integrin alpha 2

Reactivity: Rat (QC Testing), Mouse (Tested in Development)

Isotype: Armenian Hamster IgG, λ

Immunogen: Cultured Rat Glomerular Epithelial

Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, Immunoprecipitation (Reported)

Concentration: 0.5 mg/ml

RRID: AB_397413

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

関連製品

Purified Hamster IgG2, λ1 Isotype Control RUO

サイズ 0.5 mg カタログ番号 553962

FITC Mouse Anti-Armenian and Syrian Hamster IgG Cocktail RUO

サイズ 0.5 mg カタログ番号 554011

554998 Rev. 12

抗体の詳細

Ha1/29

The Ha1/29 monoclonal antibody specifically binds to intergrin α2 chain (CD49b or VLA-2 α chain), the 160-kDa transmembrane glycoprotein that non-covalently associates with the intergrin β1 subunit (CD29) to form the intergrin α2β1 complex known as VLA-2. VLA-2, a receptor for collagen and laminin, is expressed on activated lymphocytes, epithelial cells, and platelets. It has been reported that Ha1/29 antibody can block VLA-2 binding to collagen and laminin. Ha1/29 antibody also reacts with mouse CD49b.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

554998 Rev. 12

フォーマットの詳細

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

フォーマット: Purified

554998 Rev.12

引用＆参考文献

View product citations for antibody "554998" on CiteAb

Development References (3)

Hemler ME. VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu Rev Immunol. 1990; 8:365-400. (Biology). View Reference
Mendrick DL, Kelly DM, duMont SS, Sandstrom DJ. Glomerular epithelial and mesangial cells differentially modulate the binding specificities of VLA-1 and VLA-2. Lab Invest. 1995; 72(3):367-375. (Clone-specific: Blocking). View Reference
Mendrick DL, Kelly DM. Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest. 1993; 69(6):690-702. (Immunogen: Blocking, Immunocytochemistry (cytospins), Immunoprecipitation). View Reference

554998 Rev. 12

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.