BD Pharmingen™ PE Rat Anti-Mouse Zbtb46
クローン U4-1374 (RUO)
Analysis of Zbtb46 Expression
Left Panel - Two-color flow cytometric analysis Zbtb46 expression in mouse splenocytes. Mouse splenocytes were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were stained with BD Horizon™ BV421 Armenian Hamster Anti-Mouse CD11c (Cat. No. 565452) and either PE Rat IgG1, κ Isotype Control (Cat. No. 554685; Left Plot) or PE Rat Anti-Mouse Zbtb46 antibody (Cat. No. 565832; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of Zbtb46 (or Ig Isotype control staining) versus CD11c were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes.
Right Panel. Multicolor flow cytometric analysis of Zbtb46 expression in mouse splenic conventional dendritic cells. Similarly fixed and permeabilized splenocytes were stained with BD Horizon™ BV421 Armenian Hamster Anti-Mouse CD11c, Alexa Fluor® 647 Rat Anti-Mouse I-A/I-E (Cat. No. 562367), and either PE Rat IgG1, κ Isotype Control (dashed line histogram) or PE Rat Anti-Mouse Zbtb46 antibody (solid line histogram). The fluorescence histogram showing Zbtb46 expression (or Ig Isotype control staining) for conventional dendritic cells (cDC) was derived from CD11c-hi I-A/I-E-hi gated events with the forward and side light-scatter characteristics of intact splenic leucocytes.
Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Analysis of Zbtb46 Expression
Left Panel - Two-color flow cytometric analysis Zbtb46 expression in mouse splenocytes. Mouse splenocytes were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were stained with BD Horizon™ BV421 Armenian Hamster Anti-Mouse CD11c (Cat. No. 565452) and either PE Rat IgG1, κ Isotype Control (Cat. No. 554685; Left Plot) or PE Rat Anti-Mouse Zbtb46 antibody (Cat. No. 565832; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of Zbtb46 (or Ig Isotype control staining) versus CD11c were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes.
Right Panel. Multicolor flow cytometric analysis of Zbtb46 expression in mouse splenic conventional dendritic cells. Similarly fixed and permeabilized splenocytes were stained with BD Horizon™ BV421 Armenian Hamster Anti-Mouse CD11c, Alexa Fluor® 647 Rat Anti-Mouse I-A/I-E (Cat. No. 562367), and either PE Rat IgG1, κ Isotype Control (dashed line histogram) or PE Rat Anti-Mouse Zbtb46 antibody (solid line histogram). The fluorescence histogram showing Zbtb46 expression (or Ig Isotype control staining) for conventional dendritic cells (cDC) was derived from CD11c-hi I-A/I-E-hi gated events with the forward and side light-scatter characteristics of intact splenic leucocytes.
Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- An isotype control should be used at the same concentration as the antibody of interest.
関連製品
The U4-1374 monoclonal antibody specifically recognizes the Zinc finger and BTB domain containing 46 (Zbtb46) transcription factor, which is also known as BTB-ZF protein expressed in effector lymphocytes (BZEL), or BTB (POZ) domain containing 4 (Btbd4). Zbtb46 is a transcription factor that is selectively expressed by conventional dendritic cells (cDC; also known as classical dendritic cells) and their committed progenitors. It is not expressed by plasmacytoid DCs (pDC), monocytes, macrophages, or other lymphoid or myeloid lineages. Although Zbtb46 is not required for cDC development, it represses the expression of alternative myeloid growth factor receptors and thereby enforces cDC lineage restriction.
Development References (3)
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Meredith MM, Liu K, Darrasse-Jeze G, et al. Expression of the zinc finger transcription factor zDC (Zbtb46, Btbd4) defines the classical dendritic cell lineage.. J Exp Med. 2012; 209(6):1153-65. (Biology). View Reference
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Meredith MM, Liu K, Kamphorst AO, et al. Zinc finger transcription factor zDC is a negative regulator required to prevent activation of classical dendritic cells in the steady state.. J Exp Med. 2012; 209(9):1583-93. (Biology). View Reference
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Satpathy AT, KC W, Albring JC, et al. Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages.. J Exp Med. 2012; 209(6):1135-52. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.