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Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
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The 22H9 monoclonal antibody specifically recognizes the Thymic stromal lymphopoietin protein receptor (TSLP Receptor or TSLPR) which is also known as Cytokine receptor-like molecule 2 (CRLM2), Lymphocyte antigen 114 (Ly114), Type I cytokine receptor delta 1, or Transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (Tpte2). This type I transmembrane glycoprotein is encoded by Crlf2 (Cytokine receptor-like factor 2) which belongs to the hematopoietin receptor gene superfamily. The functional, high affinity receptor complex for Thymic stromal lymphopoietin (TSLP) is comprised of the TSLP Receptor and Interleukin-7 receptor alpha chain (IL-7Rα/CD127). TSLP is produced by a variety of cell types including epithelial cells, keratinocytes, and stromal cells. The TSLP Receptor is variably expressed on epithelial cells and on various cell types of hematopoietic origin including thymocytes, T cells, NK cells, B cells, monocytes, macrophages, dendritic cells (DC), granulocytes, and mast cells. TSLP signaling through the heterodimeric TSLP Receptor complex induces Stat5 tyrosine phosphorylation and supports the maturation and differentiation of multiple different cells types including B and T cells as well as dendritic cells that promote type-2 (Th2-like) T cell responses.
Development References (6)
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Isaksen DE, Baumann H, Zhou B, et al. Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction.. J Immunol. 2002; 168(7):3288-94. (Clone-specific: Flow cytometry). View Reference
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Iseki M, Omori-Miyake M, Xu W, et al. Thymic stromal lymphopoietin (TSLP)-induced polyclonal B-cell activation and autoimmunity are mediated by CD4+ T cells and IL-4.. Int Immunol. 2012; 24(3):183-95. (Clone-specific: Flow cytometry). View Reference
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Kashyap M, Rochman Y, Spolski R, Samsel L, Leonard WJ. Thymic stromal lymphopoietin is produced by dendritic cells. J Immunol. 2011; 187(3):1207-1211. (Biology). View Reference
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Park LS, Martin U, Garka K, et al. Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. J Exp Med. 2000; 192(5):659-670. (Biology). View Reference
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Rochman Y, Leonard WJ. The role of thymic stromal lymphopoietin in CD8+ T cell homeostasis. J Immunol. 2008; 181(11):7699-7705. (Clone-specific: Flow cytometry). View Reference
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Takai T. TSLP expression: cellular sources, triggers, and regulatory mechanisms. Allergol Int. 2012; 61(1):3-17. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
