製品詳細
BD Pharmingen™
Il27ra; IL-27-R; IL-27Rα; T cell cytokine receptor; TCCR; WSX-1; CRL1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Mouse TSRR Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738753
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 ML
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 ML
カタログ番号
554657
Lysing Buffer RUO
サイズ
100 ML
カタログ番号
555899
PE Rat IgG2a, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
554689
FITC Hamster Anti-Mouse CD3e RUO
サイズ
0.5 mg
カタログ番号
553062
FITC Hamster Anti-Mouse CD3e RUO
サイズ
0.1 mg
カタログ番号
553061
564337 Rev. 1
抗体の詳細
2918
The 2918 monoclonal antibody specifically binds to the alpha subunit (IL-27 Receptor alpha/IL-27 Rα) of the mouse Interleukin-27 Receptor complex. IL-27 Rα is a type I transmembrane glycoprotein that belongs to the type I cytokine receptor family. It is also known as WSX-1 and T cell cytokine receptor (TCCR). IL-27 Rα combines with gp130 (CD130) to form a high-affinity cell surface receptor that transduces intracellular signals through the JAK-STAT pathway upon IL-27 binding. IL-27 is a heterodimeric cytokine that is composed of the Epstein-Barr virus-induced gene 3 (EBI3) and IL-27p28 (IL-30) protein subunits. This multifunctional cytokine is produced by activated macrophages, dendritic cells, and epithelial cells. It mediates a broad array of pro- and anti-inflammatory responses. The IL-27 Receptor is expressed by a variety of cell types including naïve T cells and B cells. It is more highly expressed by NK cells and subsets of activated T cells.
564337 Rev. 1
フォーマットの詳細
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
564337 Rev.1
引用＆参考文献
View product citations for antibody "564337" on CiteAb
Development References (2)
-
Hunter CA, Kastelein R. Interleukin-27: balancing protective and pathological immunity. Immunity. 2012; 37(6):960-969. (Biology). View Reference
-
Villarino AV, Larkin J, 3rd, et al. Positive and negative regulation of the IL-27 receptor during lymphoid cell activation. J Immunol. 2005; 174(12):7684-7691. (Immunogen: ELISA, Flow cytometry). View Reference
564337 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.