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PE Rat Anti-Mouse CCRL2
PE Rat Anti-Mouse CCRL2

Multiparameter analysis of CCRL2 expression by mouse peritoneal mast cells. Peritoneal cells were obtained from untreated mice and were stained with BV421 Rat Anti-Mouse CD117 (Cat. No. 562609) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 554689; dashed line histogram) or PE Rat Anti-Mouse CCRL2 (Cat. No. 564946; solid line histogram). Fluorescence histograms were derived from CD117+ gated events with forward and side-light-scattering characteristics of viable mouse peritoneal mast cells. Flow cytometry was performed on a BD FACSCanto™ II.

Multiparameter analysis of CCRL2 expression by mouse peritoneal mast cells. Peritoneal cells were obtained from untreated mice and were stained with BV421 Rat Anti-Mouse CD117 (Cat. No. 562609) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 554689; dashed line histogram) or PE Rat Anti-Mouse CCRL2 (Cat. No. 564946; solid line histogram). Fluorescence histograms were derived from CD117+ gated events with forward and side-light-scattering characteristics of viable mouse peritoneal mast cells. Flow cytometry was performed on a BD FACSCanto™ II.

製品詳細
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BD Pharmingen™
C-C chemokine receptor-like 2; L-CCR; Lccr; Cmkbr1l2; Ackr5
Mouse (QC Testing)
Rat IgG2a, κ
Mouse CCRL2 Peptide
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739023
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564946 Rev. 2
抗体の詳細
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BZ2E3

The BZ2E3 monoclonal antibody specifically binds to CCRL2 (C-C chemokine receptor-like 2) which is also known as Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR/Lccr). CCRL2 is a chemokine receptor-like, 7-transmembrane protein that can bind CCL19 or the nonchemokine chemoattractant, chemerin/RARRES2. CCRL2 is expressed by a variety of cell types including mast cells, macrophages and some endothelial cells. CCRL2 appears to play various roles in regulating chemokine activities, e.g., by binding and internalizing excess local levels of the CCL19/MIP-3β chemokine. Alternatively, endothelial cell-expressed CCRL2 can bind and present the chemerin/RARRES2 chemoattractant to lymphoid cells that express the functional signaling receptor, CMKLR1, and promote their adhesion.

564946 Rev. 2
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564946 Rev.2
引用&参考文献
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Development References (4)

  1. Monnier J, Lewen S, O'Hara E, et al. Expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells. J Immunol. 2012; 189(2):956-967. (Clone-specific: Flow cytometry). View Reference
  2. Shimada T, Matsumoto M, Tatsumi Y, Kanamaru A, Akira S. A novel lipopolysaccharide inducible C-C chemokine receptor related gene in murine macrophages. FEBS Lett. 1998; 425(3):490-494. (Biology). View Reference
  3. Yoshimura T, Oppenheim JJ. Chemokine-like receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2); two multifunctional receptors with unusual properties. Exp Cell Res. 2011; 317(5):674-684. (Biology). View Reference
  4. Zabel BA, Nakae S, Zúñiga L, et al. Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J Exp Med. 2008; 205(10):2207-2220. (Immunogen: Blocking, ELISA, Flow cytometry). View Reference
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564946 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.