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PE Mouse Anti-Pig CD8a
PE Mouse Anti-Pig CD8a

CD8 expression on peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE-conjugated 76-2-11 and FITC-conjugated anti-pig CD4a 74-12-4 (Cat. No. 559585) monoclonal antibodies. Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), nonviable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

CD8 expression on peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE-conjugated 76-2-11 and FITC-conjugated anti-pig CD4a 74-12-4 (Cat. No. 559585) monoclonal antibodies. Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), nonviable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

製品詳細
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BD Pharmingen™
Pig (QC Testing)
Mouse BALB/c IgG2a, κ
dd miniature swine thymocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_397278
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559584 Rev. 5
抗体の詳細
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76-2-11

The 76-2-11 (also known as clone PT8) monoclonal antibody specifically binds to an epitope on the CD8α chain, a 35-kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and β chain. Cells which express the CD8αα homodimer display dimmer staining with mAb 76-2-11 than CDαβ-expressing cells. The 76-2-11 mAb does not cross-react with human or bovine cells. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4- CTL effectors/precursors and CD8-dull CD4+ T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1, but not CD3, CD4, CD5, or CD6. mAb 76-2-11 has been reported to partially inhibit in vitro cytotoxic activity of PBL to allogeneic leukocytes, but not NK-cell-mediated lysis, and to deplete CD8+ T cells in vivo. This clone was clustered as anti-CD8a at the First International Swine CD workshop.

559584 Rev. 5
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
559584 Rev.5
引用&参考文献
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Development References (12)

  1. Dato ME, Kim YB. Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells. J Immunol. 1990; 144(11):4452-4462. (Biology). View Reference
  2. Pescovitz MD, Lowman MA, Sachs DH. Expression of T-cell associated antigens by porcine natural killer cells. Immunology. 1988; 65(2):267-271. (Biology). View Reference
  3. Pescovitz MD, Lunney JK, Sachs DH. Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells. J Immunol. 1985; 134(1):37-44. (Clone-specific). View Reference
  4. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984; 133(1):368-375. (Immunogen). View Reference
  5. Saalmuller A, Aasted B, Canals A, et al. Analyses of mAb reactive with porcine CD8. Vet Immunol Immunopathol. 1994; 43(1-3):249-254. (Clone-specific). View Reference
  6. Saalmuller A, Hirt W, Maurer S, Weiland E. Discrimination between two subsets of porcine CD8+ cytolytic T lymphocytes by the expression of CD5 antigen. Immunology. 1994; 81(4):578-583. (Biology). View Reference
  7. Saalmuller A, Pauly T, Hohlich BJ, Pfaff E. Characterization of porcine T lymphocytes and their immune response against viral antigens. J Biotechnol. 1999; 73(2-3):223-233. (Biology). View Reference
  8. Smith CV, Sablinski T, Arn JS, et al. In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine. J Immunother Emphasis Tumor Immunol. 1994; 16(2):105-114. (Biology). View Reference
  9. Suzuki T, Sundt TM 3rd, Mixon A, Sachs DH. In vivo treatment with antiporcine T cell antibodies. Transplantation. 1990; 50(1):76-81. (Biology). View Reference
  10. Waters WR, Hontecillas R, Sacco RE, et al. Antigen-specific proliferation of porcine CD8alphaalpha cells to an extracellular bacterial pathogen. Immunology. 2000; 101(3):333-341. (Clone-specific). View Reference
  11. Zuckermann FA, Pescovitz MD, Aasted B, et al. Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop. Vet Immunol Immunopathol. 1998; 60(3-4):291-303. (Clone-specific). View Reference
  12. Zuckermann FA. Extrathymic CD4/CD8 double positive T cells. Vet Immunol Immunopathol. 1999; 72(1-2):55-66. (Biology). View Reference
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559584 Rev. 5

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.