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PE Mouse Anti-Pig CD4a
PE Mouse Anti-Pig CD4a

CD4 expression on peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE-conjugated 74-12-4 and FITC-conjugated anti-pig CD3å BB23-8E6-8C8 (Cat. no. 559582) monoclonal antibodies. Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. no. 555816/555815), and lymphocytes were identified by scatter profile. Flow  cytometry was performed on a BD FACSCalibur™ flow cytometry system.

CD4 expression on peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE-conjugated 74-12-4 and FITC-conjugated anti-pig CD3å BB23-8E6-8C8 (Cat. no. 559582) monoclonal antibodies. Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. no. 555816/555815), and lymphocytes were identified by scatter profile. Flow  cytometry was performed on a BD FACSCalibur™ flow cytometry system.

製品詳細
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BD Pharmingen™
Pig (QC Testing), Chicken (Tested in Development)
Mouse BALB/c IgG2b, κ
dd miniature swine thymocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_397280
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559586 Rev. 5
抗体の詳細
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74-12-4

The 74-12-4 (also known as clone PT4) monoclonal antibody specifically binds to CD4, a 55-kDa antigen expressed on T lymphocytes. This antibody does not react with CTL effectors, CTL precursors, or NK cells (ie, CD8[bright] cells) and it does not cross-react with human or bovine cells. Two peripheral T-helper lymphocyte phenotypes can be distinguished in the pig: CD4+CD8- and CD4+CD8[dull]. mAb 74-12-4 has been reported to inhibit proliferative responses of peripheral blood lymphocytes to mitogen, soluble antigen, and alloantigen. It is only marginally effective for in vivo depletion of peripheral CD4+ T cells. Two alloantigenic forms of CD4 have been recognized in miniature swine based upon their recognition (CD4.1) or lack of recognition (CD4.2) by mAb 74-12-4; the CD4.2 phenotype displays an autosomal recessive, non-MHC-linked, pattern of inheritance.  The molecular basis for the polymorphism is a cluster of  nucleotide differences leading to multiple amino-acid substitutions in the Ig CDR2-like loop structure. This mAb was clustered as anti-CD4a at the First International Swine CD Workshop.  It has been reported to crossreact with chicken leukocytes.

559586 Rev. 5
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
559586 Rev.5
引用&参考文献
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Development References (10)

  1. Brodersen R, Bijlsma F, Gori K. Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species. Vet Immunol Immunopathol. 1998; 64(1):1-13. (Biology). View Reference
  2. Dato ME, Kim YB. Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells. J Immunol. 1990; 144(11):4452-4462. (Immunogen). View Reference
  3. Gustafsson K, Germana S, Sundt TM, Sachs DH, LeGuern C. Extensive allelic polymorphism in the CDR2-like region of the miniature swine CD4 molecule. J Immunol. 1993; 151(3):1365-1370. (Biology). View Reference
  4. Pescovitz MD, Lunney JK, Sachs DH. Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells. J Immunol. 1985; 134(1):37-44. (Biology). View Reference
  5. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984; 133(1):368-375. (Biology). View Reference
  6. Saalmuller A, Aasted B, Canals A, et al. Analyses of mAb reactive with porcine CD8. Vet Immunol Immunopathol. 1994; 43(1-3):249-254. (Biology). View Reference
  7. Saalmüller A, Aasted B, Canals A. Summary of workshop findings for porcine T-lymphocyte antigens. Vet Immunol Immunopathol. 1994; 43(1-3):219-228. (Biology). View Reference
  8. Smith CV, Sablinski T, Arn JS, et al. In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine. J Immunother Emphasis Tumor Immunol. 1994; 16(2):105-114. (Biology). View Reference
  9. Sundt TM, LeGuern C, Germana S. Characterization of a polymorphism of CD4 in miniature swine. J Immunol. 1992; 148(10):3195-3201. (Biology). View Reference
  10. Suzuki T, Sundt TM 3rd, Mixon A, Sachs DH. In vivo treatment with antiporcine T cell antibodies. Transplantation. 1990; 50(1):76-81. (Biology). View Reference
すべて表示する (10) 表示項目を減らす
559586 Rev. 5

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.