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PE Mouse Anti-Mouse Qa-1(b)
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PE Mouse Anti-Mouse Qa-1(b)
Flow cytometric analysis of Qa-1[b] expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J (Qa-1[a]; Left Panel) or C57BL/6 (Qa-1[b]; Right Panel) mouse were stimulated for 3 days in culture with lipopolysaccharide (LPS). The cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histograms) or PE Mouse Anti-Mouse Qa-1[b] antibody (Cat. No. 566640/566641; solid line histograms) at 1 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histograms showing Qa-1[b] expression (or Ig isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative), activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of Qa-1[b] expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J (Qa-1[a]; Left Panel) or C57BL/6 (Qa-1[b]; Right Panel) mouse were stimulated for 3 days in culture with lipopolysaccharide (LPS). The cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histograms) or PE Mouse Anti-Mouse Qa-1[b] antibody (Cat. No. 566640/566641; solid line histograms) at 1 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histograms showing Qa-1[b] expression (or Ig isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative), activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
製品詳細
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BD Pharmingen™
H2-Qa1b; H2-T23; Qa-1(b); Qa-1b; Qa1b; T23b
Mouse (QC Testing)
Mouse B6-Tla(a), also known as B6-Tlaª or QA-1ª IgG1, κ
Qa-1[b] aa. 161-179 Peptide
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869801
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

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Stain Buffer (FBS) RUO
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Stain Buffer (BSA) RUO
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Lysing Buffer RUO
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PE Mouse IgG1, κ Isotype Control RUO
サイズ 0.1 mg カタログ番号 554680
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566641 Rev. 1
抗体の詳細
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6A8.6F10.1A6

The 6A8.6F10.1A6 monoclonal antibody reacts with the Qa-1[b] alloantigen, which is a nonclassical MHC class I (Class Ib) molecule encoded by the T23 gene of the H-2 complex (H2-T23). Qa-1 associates with β2-microglobulin and is expressed at low levels on most leukocytes and many other cell types. Its level of cell-surface expression is upregulated by IFNγ or specific peptides. Qa-1 is an oligomorphic molecule which presents a limited pool of peptides to T lymphocytes bearing αβ and γδTCR and binds to a large subpopulation of NK cells. In fact, Qa-1[b] is the ligand for CD94/NKG2A, CD94/NKG2C, and CD94/NKG2E receptors, which are expressed on NK cells. Furthermore, it has been reported that Qa-1 expressed on activated B lymphocytes is involved in immunoregulation by inducing T-cell-mediated suppression of antibody responses. The 6A8.6F10.1A6 mAb can detect Qa-1[b] on activated splenocytes from C57BL/6 and BALB/c mice (both Qa-1[b]), but not from A/j mice (Qa-1[a]), and it can block target cell recognition by CTLs specific for Qa-1-presented antigen.

        

566641 Rev. 1
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566641 Rev.1
引用&参考文献
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Development References (7)

  1. Lo WF, Ong H, Metcalf ES, Soloski MJ. T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules. J Immunol. 1999; 162(9):5398-5406. (Immunogen: Blocking). View Reference
  2. Noble A, Zhao ZS, Cantor H. Suppression of immune responses by CD8 cells. II. Qa-1 on activated B cells stimulates CD8 cell suppression of T helper 2 responses. J Immunol. 1998; 160(2):566-571. (Biology). View Reference
  3. Salcedo M, Bousso P, Ljunggren HG, Kourilsky P, Abastado JP. The Qa-1b molecule binds to a large subpopulation of murine NK cells. Eur J Immunol. 1998; 28(12):4356-4361. (Biology). View Reference
  4. Sivakumar PV, Gunturi A, Salcedo M, et al. Cutting edge: expression of functional CD94/NKG2A inhibitory receptors on fetal NK1.1+Ly-49- cells: a possible mechanism of tolerance during NK cell development. J Immunol. 1999; 162(12):6976-6980. (Biology). View Reference
  5. Soloski MJ, DeCloux A, Aldrich CJ, Forman J. Structural and functional characteristics of the class IB molecule, Qa-1. Immunol Rev. 1995; 147:67-89. (Biology). View Reference
  6. Vance RE, Jamieson AM, Raulet DH. Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J Exp Med. 1999; 190(12):1801-1812. (Biology). View Reference
  7. Vance RE, Kraft JR, Altman JD, Jensen PE, Raulet DH. Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b). J Exp Med. 1998; 188(10):1841-1848. (Biology). View Reference
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566641 Rev. 1

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