Flow cytometric analysis of human Sox7 expression in 293-F cells. Untransfected (dashed line histogram) and SOX7-transfected (solid line histogram) 293-F cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574). The fixed cells were then stained with PE Mouse Anti-Human Sox7 monoclonal antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
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Flow cytometric analysis of human Sox7 expression in 293-F cells. Untransfected (dashed line histogram) and SOX7-transfected (solid line histogram) 293-F cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574). The fixed cells were then stained with PE Mouse Anti-Human Sox7 monoclonal antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
製品詳細
BD Pharmingen™
Transcription factor SOX-7, SOX7
Human (QC Testing)
Mouse IgG1, κ
Human Sox7 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_2738896
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
564695 Rev. 1
抗体の詳細
S5-1216
The S5-1216 monoclonal antibody specifically binds to human Sox7, a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox7 plays a key role in cardiac, hematopoietic and vascular development. Sox7 is also involved in endoderm lineage determination, where Sox7 is expressed in the extraembryonic endoderm but not in the definitive endoderm. Over-expression of Sox7 in human embryonic stem cells leads to differentiation into lineage restricted extraembryonic endoderm progenitors. Furthermore, Sox7 has a tumor suppressive activity, as indicated by its down-regulation in colon, prostate, lung and breast cancer.
564695 Rev. 1
フォーマットの詳細
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564695 Rev.1
引用＆参考文献
Development References (5)
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Costa G, Mazan A, Gandillet A, Pearson S, Lacaud G, Kouskoff V. SOX7 regulates the expression of VE-cadherin in the haemogenic endothelium at the onset ofhaematopoietic development. Development. 2012; 139(9):1587-1598. (Biology). View Reference
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Gandillet A, Serrano AG, Pearson S, Lie-A-Ling M, Lacaud G, Kouskoff V. Sox7-sustained expression alters the balance between proliferation and differentiation of hematopoietic progenitors at the onset of blood specification. Blood. 2009; 114(23):4813-4822. (Biology). View Reference
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Séguin CA, Draper JS, Nagy A, Rossant J. Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells. Cell Stem Cell. 2008; 3(2):182-185. (Biology). View Reference
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Takash W, Cañizares J, Bonneaud N, et al. SOX7 transcription factor: sequence, chromosomal localisation, expression, transactivation and interference with Wnt signalling. Nucleic Acids Res. 2001; 29(21):4274-4283. (Biology). View Reference
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Zhang Y, Huang S, Dong W, et al. SOX7, down-regulated in colorectal cancer, induces apoptosis and inhibits proliferation of colorectal cancer cells. Cancer Lett. 2009; 277(1):29-37. (Biology). View Reference
564695 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
