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      PE Mouse anti-Human Fcµ Receptor
      PE Mouse anti-Human Fcµ Receptor
      Two-color flow cytometric analysis of Fcμ Receptor expression on human peripheral blood mononuclear cells. PBMC were stained with PE Mouse Anti-Human Fcµ Receptor (Cat. No. 563019), PerCP-Cy™5.5 Mouse Anti-Human CD3 (560835), and APC-Cy™7 Mouse Anti-Human CD11b (557754/560914) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of CD3 (Left Panel) or CD11b (Right Panel) versus Fcμ Receptor for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      PE Mouse anti-Human Fcµ Receptor
      Two-color flow cytometric analysis of Fcμ Receptor expression on human peripheral blood mononuclear cells. PBMC were stained with PE Mouse Anti-Human Fcµ Receptor (Cat. No. 563019), PerCP-Cy™5.5 Mouse Anti-Human CD3 (560835), and APC-Cy™7 Mouse Anti-Human CD11b (557754/560914) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of CD3 (Left Panel) or CD11b (Right Panel) versus Fcμ Receptor for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
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      BD Pharmingen™
      FcμR; FcmR; Fc mu receptor; IgM Fc receptor; FAIM3; TOSO
      Human (QC Testing)
      Mouse BALB/c IgG2b, κ
      Human IgM Fc Receptor Transfected Cell Line
      Flow cytometry (Routinely Tested)
      5 µl
      AB_2737955
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563019 Rev. 1
      抗体の詳細
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      HM7-10

      The HM7-10 monoclonal antibody specifically binds to the FAIM3-encoded high affinity receptor for IgM (also known as, Fcµ Receptor, FcµR, Fc mu receptor, Immunoglobulin mu Fc receptor, FcmR, IgM Fc receptor). The FcµR is a type 1 transmembrane glycoprotein that contains an extracellular Ig-like domain with sequence similarities to Fcα/µR and pIgR. It is likewise known as Fas apoptotic inhibitory molecule 3, TOSO or Regulator of Fas-induced apoptosis Toso based on its capacity to counter apoptosis mediated by Fas/CD95 (or TNF) in some experimental model systems. FcµRs are expressed by B cells, CD4+ T cells, CD8+ T cells, and NK cells and are highly expressed on Chronic Lymphocytic Leukemia cells. FcµRs may play roles in cellular activation, antigen presentation and IgM-dependent cell-mediated cytotoxicity. The relatively long cytoplasmic tail of FcµR contains several conserved tyrosine and serine residues. These get phosphorylated after FcµR ligation with IgM immune complexes and may initiate signaling responses. Lymphocyte FcµRs can mediate the internalization of IgM-bound antigens including microbial and viral antigens. This in turn may cause cellular activation due to subsequent antigen processing and exposure of activating ligands to intracellular receptors including Toll-like receptors. The HM7-10 antibody reportedly recognizes an epitope near the IgM ligand binding site of the FcµR whereas, a related FcµR-specific antibody, HM14-1 antibody does not. The HM7 epitope is sensitive to IgM ligand binding.

      563019 Rev. 1
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      563019 Rev.1
      引用＆参考文献
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      View product citations for antibody "563019" on CiteAb

      Development References (5)

      1. Hitoshi Y, Lorens J, Kitada SI, et al. Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells. Immunity. 1998; 8(4):461-471. (Biology). View Reference
      2. Kubagawa H, Oka S, Kubagawa Y, et al. Identity of the elusive IgM Fc receptor (FcmuR) in humans.. J Exp Med. 2009; 206(12):2779-2793. (Immunogen: Flow cytometry, Immunoprecipitation, Western blot). View Reference
      3. Shima H, Takatsu H, Fukuda S, et al. Identification of TOSO/FAIM3 as an Fc receptor for IgM.. Int Immunol. 2010; 22(3):149-156. (Biology). View Reference
      4. Vire B, David A, Wiestner A. TOSO, the Fcμ receptor, is highly expressed on chronic lymphocytic leukemia B cells, internalizes upon IgM binding, shuttles to the lysosome, and is downregulated in response to TLR activation. J Immunol. 2011; 187(8):4040-4050. (Biology). View Reference
      5. Wines BD, Trist HM, Farrugia W, et al. A conserved host and pathogen recognition site on immunoglobulins: structural and functional aspects. Adv Exp Med Biol. 2012; 946:87-112. (Biology). View Reference
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      563019 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.