PE Mouse Anti-Human CD209

BD Pharmingen™ PE Mouse Anti-Human CD209

Name: PE Mouse Anti-Human CD209
Brand: BD Pharmingen™
SKU: 551265

クローン DCN46

(RUO)

Flow cytometric analysis of CD209 expression on human monocyte derived dendritic cells. Dendritic cells were stained with either PE Mouse IgG2b κ Isotype Control (Cat. No. 555743; dashed line histogram) or PE Mouse Anti-Human CD209 (Cat. No. 551265/561765; solid line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable dendritic cells.

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製品詳細

Brand: BD Pharmingen™

Alternative Name: DC-SIGN

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2b, κ

Immunogen: Human Monocyte Derived DC Cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_394123

Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Lysing Buffer RUO

サイズ 100 mL カタログ番号 555899

Lysing Solution 10X Concentrate RUO (GMP)

サイズ 100 mL カタログ番号 349202

PE Mouse IgG2b κ Isotype Control RUO

サイズ 100 Tests カタログ番号 555743

PE Mouse Anti-Human CD209 RUO

サイズ 25 Tests カタログ番号 561765

詳細を表示

551265 Rev. 6

抗体の詳細

DCN46

The DCN46 antibody specifically binds to dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN or CD209), a type-II membrane protein of approximately 44 kDa with a mannose-binding C-type lectin domain. It is highly expressed on dendritic cells in mucosal tissues. Its sequence is identical to the HIV-1 envelope gp120-binding C-type lectin, and reports suggest that DC-SIGN binds to HIV-1 gp120 and effectively transmits infectious HIV-1 to resting T lymphocytes expressing CD4 and chemokine receptors. The C-type lectin domain of DC-SIGN is also capable of binding other pathogenic viruses, bacteria, and parasites. Reports also suggest that DC-SIGN enables the highly efficient migration of dendritic cells from blood into the tissues. It can interact with ICAM-2, which has a similar sequence as ICAM-3, and is abundantly expressed on vascular and lymphoid endothelium. Thus, DC-SIGN mediates dendritic cells rolling and transendothelial migration, and its interaction with ICAM-2 is essential to specific migratory functions of dendritic cells.

551265 Rev. 6

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 488 nm, 532 nm, 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

551265 Rev.6

引用＆参考文献

View product citations for antibody "551265" on CiteAb

Development References (4)

Geijtenbeek TBH, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1 -binding protein that enhances trans-infection of T cells. Cell. 2000; 100(5):587-597. (Biology). View Reference
Geijtenbeek TBH, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000; 100(5):575-585. (Biology). View Reference
Sallusto F, Cella M, Danieli C, Lanzavecchia A. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products. J Exp Med. 1995; 182(2):389-400. (Immunogen). View Reference
Steinman RM. DC-SIGN: a guide to some mysteries to dendritic cells. Cell. 2000; 100(5):491-494. (Biology). View Reference

すべて表示する (4)

551265 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.