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PE Mouse Anti-Human CCL22
製品詳細
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BD Pharmingen™
C-C motif chemokine 22; STCP-1; SCYA22; MDC; MDC(1-69); ABCD-1, DC/B-CK
Human (QC Testing)
Mouse IgG1, κ
Human CCL22 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739414
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
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565950 Rev. 1
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T51-719

The T51-719 monoclonal antibody specifically recognizes C-C motif chemokine 22 (CCL22), which is also known as Macrophage-derived chemokine (MDC), Stimulated T-cell chemotactic protein 1 (STCP-1), or Small-inducible cytokine A22 (SCYA22). CCL22 is produced by macrophages, dendritic cells, B cells, and NK cells. CCL22 binds to and signals through the cell surface receptor, CCR4. It attracts chronically activated T cells, including cutaneous lymphocyte antigen (CLA)-positive T cells, Th2-like cells, and regulatory T cells into inflammatory sites. CCL22 also attracts NK cells, monocytes/macrophages, and dendritic cells.

565950 Rev. 1
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
496 nm, 566 nm
576 nm
565950 Rev.1
引用＆参考文献
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View product citations for antibody "565950" on CiteAb

Development References (8)

  1. Chang Ms, McNinch J, Elias C, et al. Molecular cloning and functional characterization of a novel CC chemokine, stimulated T cell chemotactic protein (STCP-1) that specifically acts on activated T lymphocytes.. J Biol Chem. 1997; 272(40):25229-37. (Biology). View Reference
  2. Ferenczi K, Fuhlbrigge RC, Pinkus J, Pinkus GS, Kupper TS. Increased CCR4 expression in cutaneous T cell lymphoma.. J Invest Dermatol. 2002; 119(6):1405-10. (Biology). View Reference
  3. Godiska R, Chantry D, Raport CJ, et al. Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells.. J Exp Med. 1997; 185(9):1595-604. (Biology). View Reference
  4. Imai T, Chantry D, Raport CJ, et al. Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J Biol Chem. 1998; 273(3):1764-1768. (Biology). View Reference
  5. Lin L, Nonoyama S, Oshiba A, Kabasawa Y, Mizutani S. TARC and MDC are produced by CD40 activated human B cells and are elevated in the sera of infantile atopic dermatitis patients.. J Med Dent Sci. 2003; 50(1):27-33. (Biology). View Reference
  6. Nakayama T, Hieshima K, Nagakubo D, et al. Selective induction of Th2-attracting chemokines CCL17 and CCL22 in human B cells by latent membrane protein 1 of Epstein-Barr virus.. J Virol. 2004; 78(4):1665-74. (Biology). View Reference
  7. Robertson MJ. Role of chemokines in the biology of natural killer cells.. J Leukoc Biol. 2002; 71(2):173-83. (Biology). View Reference
  8. Vulcano M, Albanesi C, Stoppacciaro A, et al. Dendritic cells as a major source of macrophage-derived chemokine/CCL22 in vitro and in vivo.. Eur J Immunol. 2001; 31(3):812-22. (Biology). View Reference
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565950 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.