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      PE-CF594 Mouse Anti-AhR
      PE-CF594 Mouse Anti-AhR
      Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either BD Horizon™ PE-CF594 Mouse IgG1, κ Isotype Control (Cat. No. 562292; Left Plot) or BD Horizon PE-CF594 Mouse Anti-AhR antibody (Cat. No. 565790; Right Plot) at 0.25 µg/test. Bottom Plots - AhR expression in mouse splenocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained with antibodies at 0.5 µg/test, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
      PE-CF594 Mouse Anti-AhR
      Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either BD Horizon™ PE-CF594 Mouse IgG1, κ Isotype Control (Cat. No. 562292; Left Plot) or BD Horizon PE-CF594 Mouse Anti-AhR antibody (Cat. No. 565790; Right Plot) at 0.25 µg/test. Bottom Plots - AhR expression in mouse splenocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained with antibodies at 0.5 µg/test, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
      製品詳細
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      BD Horizon™
      AHR; Aryl hydrocarbon R; Ah-Receptor; Aromatic hydrocarbon receptor; bHLHe7
      Human (QC Testing), Mouse (Tested in Development)
      Mouse IgG1, κ
      Human AhR Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      AB_2869714
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
      6. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
      7. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. CF™ is a trademark of Biotium, Inc.
      10. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      11. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      12. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      13. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      565790 Rev. 1
      抗体の詳細
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      T49-550

      The T49-550 monoclonal antibody recognizes the human Aryl Hydrocarbon Receptor (AhR), which is also known as Aromatic Hydrocarbon Receptor, and cross-reacts with the mouse AhR. AhR is a member of basic helix-loop-helix transcription factors (Class E basic helix-loop-helix protein 76, bHLHe76). AhR can bind to a variety of endogenous and exogenous ligands including tryptophan metabolites, bacterial pigments, plant flavonoids, synthetic polycyclic aromatic hydrocarbons, or dioxin-like compounds. Upon ligand binding, cytoplasmic AhR undergoes conformational changes and translocates into the nucleus to induce gene transcription. AhR is widely expressed in mammalian tissues with the highest expression levels in the liver and lung. A number of studies suggest that AhR regulates inflammation mainly by attenuating the production of inflammatory cytokines by dendritic cells and macrophages.  In addition, AhR can regulate the expansion or effector functions of Th17 cells, IL-10-secreting regulatory T (Tr1) cells, and subsets of intraepithelial lymphocytes (IEL) and innate lymphoid cells (ILC), keratinocytes, and Langerhans cells.

      This antibody is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg, 610/20-nm filter).

      565790 Rev. 1
      フォーマットの詳細
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      PE-CF594
      BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE-CF594
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      615 nm
      565790 Rev.1
      引用＆参考文献
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      Development References (9)

      1. Denison MS, Nagy SR. Activation of the aryl hydrocarbon receptor by structurally diverse exogenous and endogenous chemicals. Annu Rev Pharmacol Toxicol. 2003; 43(309):334. (Biology). View Reference
      2. Gonzalez FJ, Fernandez-Salguero P. The aryl hydrocarbon receptor: studies using the AHR-null mice. Drug Metab Dispos. 1998; 26(12):1194-1198. (Biology). View Reference
      3. Henry EC, Bemis JC, Henry O, Kende AS, Gasiewicz TA. A potential endogenous ligand for the aryl hydrocarbon receptor has potent agonist activity in vitro and in vivo. Arch Biochem Biophys. 2006; 450(1):67-77. (Biology). View Reference
      4. Ho PP, Steinman L. The aryl hydrocarbon receptor: a regulator of Th17 and Treg cell development in disease. Cell Res. 2008; 18(6):605-608. (Biology). View Reference
      5. Ramsay G, Cantrell D. Environmental and metabolic sensors that control T cell biology. Front Immunol. 2015; 6(1):8. (Biology). View Reference
      6. Simones T, Shepherd DM. Consequences of AhR activation in steady-state dendritic cells. Toxicology. 2011; 119(2):293-307. (Biology). View Reference
      7. Stockinger B, Di Meglio P, Gialitakis M, Duarte JH. The aryl hydrocarbon receptor: multitasking in the immune system. Annu Rev Immunol. 2014; 32(403):432. (Biology). View Reference
      8. Veldhoen M, Hirota K, Christensen J, O'Garra A, Stockinger B. Natural agonists for aryl hydrocarbon receptor in culture medium are essential for optimal differentiation of Th17 T cells. J Exp Med. 2009; 206(1):43-49. (Biology). View Reference
      9. Volchenkov R, Nygaard V, Sener Z, Skålhegg BS. Th17 Polarization under Hypoxia Results in Increased IL-10 Production in a Pathogen-Independent Manner.. Front Immunol. 2017; 8:698. (Clone-specific: Flow cytometry). View Reference
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      565790 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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