The 25-9-17 monoclonal antibody specifically recognizes the β chain of the I-A[b] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[q] haplotypes. Reactivity with other haplotypes (eg, a, f, g7, k, r, s) has not been observed. The strain distribution of the antigen recognized by this reagent is similar or identical to that of anti-I-A[d] (Aβ[d]) mAb 34-5-3.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.