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FITC Mouse Anti-Human CD23
FITC Mouse Anti-Human CD23

Flow cytometric analysis of CD23 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse Anti-Human CD23 antibody (Cat. No. 561146; Left Panel) or with a FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; Right Panel) and Alexa Fluor® 700 Mouse anti-Human CD19 (Cat. No. 557921). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The two-color flow cytometric dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of CD23 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse Anti-Human CD23 antibody (Cat. No. 561146; Left Panel) or with a FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; Right Panel) and Alexa Fluor® 700 Mouse anti-Human CD19 (Cat. No. 557921). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The two-color flow cytometric dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

製品詳細
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BD Pharmingen™
FCER2; FcεRII; Low affinity immunoglobulin epsilon Fc receptor; BLAST-2
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
5 µl
V CD23.15
AB_10611730
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
561146 Rev. 1
抗体の詳細
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M-L233

The M-L233 antibody specifically binds to human CD23, the low affinity receptor for human IgE (FcεRII). CD23 is a type II membrane glycoprotein that is expressed by B cells, monocytes, macrophages, eosinophils, platelets and dendritic cells. CD23 mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. Soluble CD23 (sCD23) can be released by CD23-positive cells as a result of proteolytic cleavage of membrane CD23. Larger fragments of sCD23 (e.g., 25-37 kDa) retain their IgE-binding capacity whereas smaller fragments (ie, ≤ 12 kDa) do not. Soluble CD23 may have immunoregulatory effects on the growth and differentiation of B cells and other cell types.

561146 Rev. 1
フォーマットの詳細
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
561146 Rev.1
引用&参考文献
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Development References (5)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Delespesse G, Hofstetter H, Sarfati M. Low-affinity receptor for IgE (FcERII, CD23) and its soluble fragments. Int Arch Allergy Immunol. 1989; 90(1):41-44. (Biology). View Reference
  3. Gordon J, Millsum MJ, Flores-Romo L, Gillis S. Regulation of resting and cycling human B lymphocytes via surface IgM and the accessory molecules interleukin-4, CD23 and CD40. Immunology. 1989; 68(4):526-531. (Biology). View Reference
  4. Saeland S, Duvert V, Moreau I, Banchereau J. Human B cell precursors proliferate and express CD23 after CD40 ligation. J Exp Med. 1993; 178(1):113-120. (Biology). View Reference
  5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
すべて表示する (5) 表示項目を減らす
561146 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.