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FITC Hamster Anti-Mouse Vβ 8.3 T-Cell Receptor
FITC Hamster Anti-Mouse Vβ 8.3 T-Cell Receptor

Two-color analysis of the expression of Vβ 8.3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC Hamster Anti-Mouse Vβ 8.3 T-Cell Receptor (Cat. No. 553663), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

Two-color analysis of the expression of Vβ 8.3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC Hamster Anti-Mouse Vβ 8.3 T-Cell Receptor (Cat. No. 553663), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

製品詳細
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BD Pharmingen™
TCR V beta 8.3; TCR Vβ8.3
Mouse (QC Testing)
Armenian Hamster IgG3, λ1
Vβ 8.3 TCR-expressing mouse Th1 clone 3.L2
Flow cytometry (Routinely Tested)
0.5 mg/ml
21608
AB_394979
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

推奨アッセイ手順

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553663 Rev. 14
抗体の詳細
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1B3.3

The 1B3.3 antibody reacts with the Vβ 8.3 T-cell Receptor (TCR), but not the Vβ 8.1 nor Vβ 8.2 TCR, of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 8.3 is one of the restricted TCR β chains expressed by NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells in vivo. Vβ 8.3 TCR-bearing T-cell clones can be activated by 1B3.3 mAb. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.

553663 Rev. 14
フォーマットの詳細
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
553663 Rev.14
引用&参考文献
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Development References (6)

  1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). View Reference
  2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
  3. Hugo P, Kappler JW, Godfrey DI, Marrack PC. Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion. J Immunol. 1994; 52(3):1022-1031. (Biology). View Reference
  4. Kersh GJ, Donermeyer DL, Frederick KE, White JM, Hsu BL, Allen PM. TCR transgenic mice in which usage of transgenic alpha- and beta-chains is highly dependent on the level of selecting ligand. J Immunol. 1998; 161(2):585-593. (Immunogen). View Reference
  5. Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology). View Reference
  6. White J, Herman A, Pullen AM, Kubo R, Kappler JW, Marrack P. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989; 56(1):27-35. (Biology). View Reference
すべて表示する (6) 表示項目を減らす
553663 Rev. 14

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.