BV650 Mouse Anti-Human Siglec-10

BD OptiBuild™ BV650 Mouse Anti-Human Siglec-10

Name: BV650 Mouse Anti-Human Siglec-10
Brand: BD OptiBuild™
SKU: 747711

クローン 5G6

(RUO)

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製品詳細

Brand: BD OptiBuild™

Alternative Name: SIGLEC10; SIG10; Siglec-like protein 2; SLG2; PRO940

Reactivity: Human (Tested in Development)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Siglec-10 Recombinant Protein

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

RRID: AB_2744261

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
Alexa Fluor® is a registered trademark of Life Technologies Corporation.

関連製品

Human BD Fc Block™ RUO

サイズ 50 µg カタログ番号 564219

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Brilliant Stain Buffer RUO

サイズ 100 Tests カタログ番号 563794

Lysing Solution 10X Concentrate RUO (GMP)

サイズ 100 mL カタログ番号 349202

Lysing Buffer RUO

サイズ 100 mL カタログ番号 555899

詳細を表示

747711 Rev. 1

抗体の詳細

5G6

The 5G6 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 10 (Siglec-10). Siglec-10 is a type I transmembrane glycoprotein encoded by SIGLEC10 that belongs to the SIGLEC (sialic acid binding Ig-like lectin) family within the immunoglobulin superfamily. The extracellular region of Siglec-10 contains five Ig-like domains including an N-terminal V-set domain that contains the sialic acid-binding site and four C2-set domains. Siglec-10 preferentially binds to glycoconjugates containing α2,3- or α2,6-linked sialic acids present on human cell surfaces and may function as an adhesion receptor. Its cytoplasmic region contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) that upon phosphorylation can bind the SH2 domains of several SH2-containing phosphatases. This CD33-related Siglec has a mouse ortholog designated Siglec-G. Siglec-10 is expressed on B cells, eosinophils, monocytes, and a subset of CD16+CD56- natural killer (NK) cells. It may play a role as an inhibitory receptor for leucocytes involved in innate and adaptive immunity. The 5G6 antibody reportedly did not crossreact with Siglecs 3, 5, 7, 8 and 9.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

747711 Rev. 1

フォーマットの詳細

BV650

The BD Horizon Brilliant Violet™ 650 (BV650) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 406-nm and an acceptor dye with an emission maximum (Em Max) at 649-nm. BV650, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 650-nm (e.g., a 660/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BV650

励起源: Violet 405 nm

最大励起波長: 406 nm

最大蛍光波長: 649 nm

747711 Rev.1

引用＆参考文献

View product citations for antibody "747711" on CiteAb

Development References (3)

Macauley MS, Crocker PR, Paulson JC. Siglec-mediated regulation of immune cell function in disease.. Nat Rev Immunol. 2014; 14(10):653-66. (Biology). View Reference
Munday J, Kerr S, Ni J, et al. Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor.. Biochem J. 2001; 355(Pt 2):489-97. (Immunogen: ELISA, Flow cytometry). View Reference
Pillai S, Netravali IA, Cariappa A, Mattoo H. Siglecs and immune regulation.. Annu Rev Immunol. 2012; 30:357-92. (Biology). View Reference

747711 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.