The M-B371 monoclonal antibody specifically recognizes CD37. CD37 is a 40-52 kDa type II integral membrane glycoprotein present on B cells from the pre-B-cell stage but not on plasma cells. CD37 is also expressed on activated, proliferating cells of germinal centers. There is low expression of CD37 on some T and myeloid cells. The function of CD37 has not yet been clearly identified.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.