The 218820 monoclonal antibody specifically recognizes CD369 which is also known as Dendritic cell-associated C-type lectin 1 (DC-associated C-type lectin 1, Dectin-1, or Dectin1), or Beta-glucan receptor (Bgr). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7 member a). CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a cytoplasmic tail with an immunoreceptor tyrosine-based activation motif (ITAM). It is expressed on monocytes/macrophages, neutrophils, and some populations of dendritic cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 (Clec7a)-mediated signaling may play roles in leucocyte responses including phagocytosis or enhanced cytokine production.
The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.