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BV421 Mouse Anti-Human CD179a (VpreB)
BV421 Mouse Anti-Human CD179a (VpreB)
Flow cytometric analysis of surface CD179a (VpreB) expression on human NALM-6 cells. Cells from the human NALM-6 (B cell precursor leukemia) cell line were surface stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No.562438; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD179a (VpreB) antibody (Cat. No. 566583; solid line histogram) at 1.0 μg/ml. The fluorescence histogram showing CD179a (VpreB) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of surface CD179a (VpreB) expression on human NALM-6 cells. Cells from the human NALM-6 (B cell precursor leukemia) cell line were surface stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No.562438; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD179a (VpreB) antibody (Cat. No. 566583; solid line histogram) at 1.0 μg/ml. The fluorescence histogram showing CD179a (VpreB) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
製品詳細
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BD Horizon™
VPREB; VPREB1; V pre β; IGVPB; pre-B lymphocyte 1; IGI; immunoglobulin iota
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human VpreB/λ5 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
VII 70514
AB_2739748
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

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For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566583 Rev. 1
抗体の詳細
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HSL96

The HSL96 monoclonal antibody specifically recognizes CD179a which is also known as VpreB (V pre beta/V pre β). CD179a (VpreB) is a ~18 kDa, Ig V domain-like protein that is encoded by VPREB1 (pre-B lymphocyte 1). This member of the immunoglobulin (Ig) gene superfamily is primarily expressed in the cytoplasm of normal pro-B and early pre-B cells and at low levels on the surface of early pre-B cells.  It is not expressed by normal mature circulating B cells or by other leucocyte populations. CD179a (VpreB) associates noncovalently with CD179b (λ5) to form CD179a/CD179b (VpreB/ λ5), an Ig light chain-like structure called the surrogate light chain. Surrogate light chains are disulfide-linked to membrane-bound IgM heavy chains in association with signal transducer CD79a/CD79b heterodimers which together form the pre-B cell receptor (preBCR) complex. The preBCR plays a critical role in early B cell proliferation and differentiation. The HSL96 antibody can reportedly be used to stain cytoplasmic and cell surface CD179a (VpreB).

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421  can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421  conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

566583 Rev. 1
フォーマットの詳細
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566583 Rev.1
引用&参考文献
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Development References (6)

  1. Karasuyama H, Lebien TW, Copper MD, Clark EC. CD179 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:112-115.
  2. Kiyokawa N, Sekino T, Matsui T, et al. Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.. Mod Pathol. 2004; 17(4):423-9. (Clone-specific: Flow cytometry). View Reference
  3. Matsuo Y, Drexler HG, Okochi A, Sugimoto A, Harashima A, Orita K. Characterization of human B cell-precursor leukemia and mature B-cell leukaemia/lymphoma cell lines: Expression and distribution of human pre-B-cell receptor. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:117-120.
  4. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Expression profile of pre B-cell receptor components in acute lymphoblastic leukemia and its application to the diagnosis and classification of the disease. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:115-117.
  5. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). View Reference
  6. Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific: Flow cytometry). View Reference
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566583 Rev. 1

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