Skip to main content Skip to navigation
BV421 Mouse Anti-Human CCR10
BV421 Mouse Anti-Human CCR10
Two-color flow cytometric analysis of human CCR10 expression on human peripheral blood lymphocytes. Whole blood was treated with BD PharmLyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. After washing, the leucocytes were then stained with FITC Mouse Anti-Human CD3 antibody (Cat No. 555332/561806/561807) and either BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; Left Panel) or BD Horizon BV421 Mouse Anti-Human CCR10 antibody (Cat. No. 564770; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of CCR10 (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light- scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of human CCR10 expression on human peripheral blood lymphocytes. Whole blood was treated with BD PharmLyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. After washing, the leucocytes were then stained with FITC Mouse Anti-Human CD3 antibody (Cat No. 555332/561806/561807) and either BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; Left Panel) or BD Horizon BV421 Mouse Anti-Human CCR10 antibody (Cat. No. 564770; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of CCR10 (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light- scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
製品詳細
Down Arrow Up Arrow


BD Horizon™
C-C CKR-10, C-C CKR-10, G-protein coupled receptor 2, CCR-10, GPR2
Human (QC Testing)
Mouse C57BL/6 IgG2a, κ
Human CCR10 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738942
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564770 Rev. 2
抗体の詳細
Down Arrow Up Arrow
1B5

The 1B5 monoclonal antibody specifically binds to CCR10 (CC-chemokine receptor 10). CCR10 is a seven transmembrane domain G-protein-coupled receptor that is also known as GPR-2 (G-protein coupled receptor 2). CCR10 is expressed by cells that mediate epithelial immunity including a small subset of T memory cells, IgA-secreting B cells, dermal endothelial cells and fibroblasts. It is also expressed on EBV-immortalized B cells. Chemokines CCL27  and CCL28 are the ligands for CCR10. CCR10 reportedly not only functions in regulating the homing and function of cells that mediate epithelial immunity. It may also regulate the epithelial localization, survival, and proliferation of tumor cells and facilitate their evasion from immune surveillance.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

564770 Rev. 2
フォーマットの詳細
Down Arrow Up Arrow
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV421
Violet 405 nm
407 nm
423 nm
564770 Rev.2
引用&参考文献
Down Arrow Up Arrow
View product citations for antibody "564770" on CiteAb

Development References (6)

  1. Homey B, Wang W, Soto H, et al. Cutting edge: the orphan chemokine receptor G protein-coupled receptor-2 (GPR-2, CCR10) binds the skin-associated chemokine CCL27 (CTACK/ALP/ILC). J Immunol. 2000; 164(7):3465-3470. (Biology). View Reference
  2. Jaimes MC, Rojas OL, Kunkel EJ, et al. Maturation and trafficking markers on rotavirus-specific B cells during acute infection and convalescence in children. J Virol. 2004; 78(20):10967-10976. (Clone-specific). View Reference
  3. Marchese A, Docherty JM, Nguyen T, et al. Cloning of human genes encoding novel G protein-coupled receptors. Genomics. 1994; 23(3):609-618. (Biology). View Reference
  4. Pan J, Kunkel EJ, Gosslar U, et al. A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues. J Immunol. 2000; 165(6):2943-2949. (Biology). View Reference
  5. Soler D, Humphreys TL, Spinola SM, Campbell JJ. CCR4 versus CCR10 in human cutaneous TH lymphocyte trafficking. Blood. 2003; 101(5):1677-1682. (Immunogen: Flow cytometry). View Reference
  6. Xiong N, Fu Y, Hu S, Xia M, Yang J. CCR10 and its ligands in regulation of epithelial immunity and diseases. Protein Sci. 2012; 3(8):571-580. (Biology). View Reference
すべて表示する (6) 表示項目を減らす
564770 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.