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      BUV496 Mouse Anti-Integrin α7 (ITGA7)
      製品詳細
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      BD OptiBuild™
      ITGA7; Integrin α7; integrin alpha-7; α7 Integrin
      Human,Mouse (Tested in Development)
      Mouse C57BL/6 IgG1, κ
      Mouse Myoblasts
      Flow cytometry (Qualified)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      推奨アッセイ手順

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

      Note:  When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed.  For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s).

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      Product Notices

      1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      2. Researchers should determine the optimal concentration of this reagent for their individual applications.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Ultraviolet 496 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
      9. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      10. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      11. For U.S. patents that may apply, see bd.com/patents.
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      755145 Rev. 2
      抗体の詳細
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      3C12

      The 3C12 monoclonal antibody specifically recognizes Integrin alpha 7 (Integrin α7 or α7 Integrin). Integrins mediate important intercellular and cell-extracellular matrix interactions in a variety of biological processes. Integrin α7 is a single-pass type I transmembrane glycoprotein that is encoded by ITGA7 (Integrin subunit alpha 7) which belongs to the Integrin alpha chain family. Integrin α7 is mainly expressed by skeletal, cardiac, and smooth muscle cells. It associates with Integrin β1 to form a heterodimeric Integrin α7β1 complex. This heterodimer functions as the major laminin-binding receptor. Integrin α7β1 mediates cellular binding to components of the extracellular matrix including laminin-1 as well as the laminin isoforms-2 and -4. Integrin α7β1 participates in the adhesion and motility of myoblasts during myogenesis and helps guide muscle fiber formation and maintenance.

      755145 Rev. 2
      フォーマットの詳細
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      BUV496
      The BD Horizon Brilliant™ Ultraviolet 496 (BUV496) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 496-nm. BUV496, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 500-nm (e.g., 515/30-nm bandpass filter). The acceptor dye can be excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BUV496
      Ultraviolet 355 nm
      350 nm
      496 nm
      755145 Rev.2
      引用＆参考文献
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      View product citations for antibody "755145" on CiteAb

      Development References (10)

      1. Burkin DJ, Kaufman SJ. The alpha7beta1 integrin in muscle development and disease.. Cell Tissue Res. 1999; 296(1):183-90. (Biology). View Reference
      2. Kobayashi N, Oda T, Takizawa M, et al. Integrin α7 and Extracellular Matrix Laminin 211 Interaction Promotes Proliferation of Acute Myeloid Leukemia Cells and Is Associated with Granulocytic Sarcoma.. Cancers (Basel). 2020; 12(2):E363. (Biology). View Reference
      3. Liu J, Burkin DJ, Kaufman SJ. Increasing alpha 7 beta 1-integrin promotes muscle cell proliferation, adhesion, and resistance to apoptosis without changing gene expression.. Am J Physiol Cell Physiol. 2008; 294(2):C627-40. (Biology). View Reference
      4. Mayer U, Saher G, Fässler R, et al. Absence of integrin alpha 7 causes a novel form of muscular dystrophy.. Nat Genet. 1997; 17(3):318-23. (Biology). View Reference
      5. Mielenz D, Hapke S, Pöschl E, von Der Mark H, von Der Mark K. The integrin alpha 7 cytoplasmic domain regulates cell migration, lamellipodia formation, and p130CAS/Crk coupling.. J Biol Chem. 2001; 276(16):13417-26. (Biology: Flow cytometry). View Reference
      6. Rosbottom A, Scudamore CL, von der Mark H, Thornton EM, Wright SH, Miller HR. TGF-beta 1 regulates adhesion of mucosal mast cell homologues to laminin-1 through expression of integrin alpha 7.. J Immunol. 2002; 169(10):5689-95. (Clone-specific: Flow cytometry, Functional assay). View Reference
      7. Schöber S, Mielenz D, Echtermeyer F, et al. The role of extracellular and cytoplasmic splice domains of alpha7-integrin in cell adhesion and migration on laminins.. Exp Cell Res. 2000; 255(2):303-13. (Immunogen). View Reference
      8. Su Y, Guan XQ, Liu FQ, Wang YL. The effects of MIBG on the invasive properties of HepG2 hepatocellular carcinoma cells.. Int J Mol Med. 2014; 34(3):842-8. (Biology). View Reference
      9. Ziober BL, Vu MP, Waleh N, Crawford J, Lin CS, Kramer RH. Alternative extracellular and cytoplasmic domains of the integrin alpha 7 subunit are differentially expressed during development.. J Biol Chem. 1993; 268(35):26773-83. (Biology). View Reference
      10. von der Mark H, Williams I, Wendler O, Sorokin L, von der Mark K, Pöschl E. Alternative splice variants of alpha 7 beta 1 integrin selectively recognize different laminin isoforms.. J Biol Chem. 2002; 277(8):6012-6. (Clone-specific: Functional assay). View Reference
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      755145 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.