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BUV395 Mouse Anti-Human CD13
製品詳細
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BD OptiBuild™
ANPEP; APN; Aminopeptidase N; Alanyl aminopeptidase; LAP1; PEPN
Human (Tested in Development)
Mouse BALB/c X C57BL/6 IgG1, κ
KG-1a Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
V MA21
290
AB_2743160
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


調製と保管

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

製品通知

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
745670 Rev. 1
抗体の詳細
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L138

The L138 monoclonal antibody (also known as Leu-M7) specifically binds to a glycosylated 150 kDa type II integral membrane zinc-metalloprotease. The CD13 antigen is also known as aminopeptidase N, APN, ANPEP, and gp150. The CD13 antigen is expressed on granulocytes, monocytes, mast cells, and granulocyte/macrophage progenitor cells (CFU-GM), but not on lymphocytes, platelets, or erythrocytes. It is expressed on most acute myeloid leukemia (AML) cells and some chronic myeloid leukemia (CML) cells. The CD13 antigen is also expressed on epithelial cells of the kidney, small intestine, and respiratory tract, as well as in synaptic membranes in the central nervous system (CNS). The CD13 antigen is involved in the metabolism of many regulatory peptides. Clustering of the CD13 antigen by various forms of ligation promotes the adhesion between monocytes and endothelial cells. The CD13 antigen is the receptor for human coronavirus 229E, the causative agent for some cases of upper respiratory infection.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

745670 Rev. 1
フォーマットの詳細
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BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV395
Ultraviolet 355 nm
348 nm
395 nm
745670 Rev.1
引用&参考文献
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開発者向け参考資料 (20)

  1. Gadd S. Cluster report: CD13. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989::782-784.
  2. Ashmun RA, Holmes KV, Shapiro LH, et al. CD13 (aminopeptidase N) cluster workshop report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995::771-775.
  3. Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E. Myeloid progenitor surface antigen identified by monoclonal antibody.. Br J Haematol. 1985; 61(1):11-20. (Biology). 参考文献を見る
  4. Büchi G, Girotto M, Baldini G, et al. Differentiation phenotypes on cells of acute myeloid leukemia studied by My7, My9, My4, Mo1 and Ja monoclonal antibodies.. Pathologica. 79(1064):699-704. (Biology). 参考文献を見る
  5. Cheson BD, Cassileth PA, Head DR, et al. Report of the National Cancer Institute-sponsored workshop on definitions of diagnosis and response in acute myeloid leukemia.. J Clin Oncol. 1990; 8(5):813-9. (Biology). 参考文献を見る
  6. Drexler HG. Classification of acute myeloid leukemias--a comparison of FAB and immunophenotyping.. Leukemia. 1987; 1(10):697-705. (Biology). 参考文献を見る
  7. Foon KA, Gale RP, Todd RF. Recent advances in the immunologic classification of leukemia.. Semin Hematol. 1986; 23(4):257-83. (Biology). 参考文献を見る
  8. Griffin JD, Ritz J, Beveridge RP, Lipton JM, Daley JF, Schlossman SF. Expression of MY7 antigen on myeloid precursor cells.. Int J Cell Cloning. 1983; 1(1):33-48. (Biology). 参考文献を見る
  9. Howard MR, Thomas L, Reid MM. Variable detection of myeloid antigens in childhood acute lymphoblastic leukaemia.. J Clin Pathol. 1994; 47(11):1006-9. (Clone-specific: Flow cytometry). 参考文献を見る
  10. Kirshenbaum AS, Goff JP, Semere T, Foster B, Scott LM, Metcalfe DD. Demonstration that human mast cells arise from a progenitor cell population that is CD34(+), c-kit(+), and expresses aminopeptidase N (CD13).. Blood. 1999; 94(7):2333-42. (Biology). 参考文献を見る
  11. Lee SH, Crocker PR, Westaby S, et al. Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters.. J Exp Med. 1988; 168(3):1193-8. (Biology). 参考文献を見る
  12. Look AT, Ashmun RA, Shapiro LH, et al. Report on the CD13 (aminopeptidase N) cluster Workshop. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:784-787.
  13. Mina-Osorio P, Winnicka B, O'Conor C, et al. CD13 is a novel mediator of monocytic/endothelial cell adhesion.. J Leukoc Biol. 2008; 84(2):448-59. (Biology). 参考文献を見る
  14. Pombo de Oliveira MS, Matutes E, Rani S, Morilla R, Catovsky D. Early expression of MCS2 (CD13) in the cytoplasm of blast cells from acute myeloid leukaemia.. Acta Haematol. 1988; 80(2):61-4. (Biology). 参考文献を見る
  15. Sakai K, Hattori T, Sagawa K, Yokoyama M, Takatsuki K. Biochemical and functional characterization of MCS-2 antigen (CD13) on myeloid leukemic cells and polymorphonuclear leukocytes.. Cancer Res. 1987; 47(21):5572-6. (Biology). 参考文献を見る
  16. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Clone-specific: Flow cytometry). 参考文献を見る
  17. Yang P, Wang X. COVID-19: a new challenge for human beings. Cell Mol Immunol. 2020; 17(5):555-557. (Biology). 参考文献を見る
  18. Yeager CL, Ashmun RA, Williams RK, et al. Human aminopeptidase N is a receptor for human coronavirus 229E.. Nature. 1992; 357(6377):420-2. (Biology). 参考文献を見る
  19. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
  20. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Clone-specific: Flow cytometry). 参考文献を見る
すべて表示する (20) 表示項目を減らす
745670 Rev. 1

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