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Biotin Mouse Anti-Mouse I-A[b]
Biotin Mouse Anti-Mouse I-A[b]

Immunohistochemical staining for mouse I-A[b]. Frozen sections of mouse spleen were stained with AF6-120.1 antibody. Cells expressing I-A[b] can be identified by the brown staining on cell membranes. Magnification 20X.

Immunohistochemical staining for mouse I-A[b]. Frozen sections of mouse spleen were stained with AF6-120.1 antibody. Cells expressing I-A[b] can be identified by the brown staining on cell membranes. Magnification 20X.

製品詳細
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BD Pharmingen™
Mouse (QC Testing)
Mouse BALB/c IgG2a, κ
C57BL/10J Mouse Splenocytes
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
62.5 µg/ml
AB_393746
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


推奨アッセイ手順

Immunohistochemistry: The AF6-120.1 clone reactive against mouse I-A[b] is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse (C57BL/6) spleen and thymus. The antibody stains the I-A[b] molecules found on the surface of cells expressing MHC-II in strains of mice expressing this haplotype. For optimal immunohistochemical staining, AF6-120.1 antibody should be titrated (1:10 to 1:50 dilution) and developed with Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone AF6-120.1 is not recommended for formalin-fixed paraffin embedded sections.

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550553 Rev. 3
抗体の詳細
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AF6-120.1

The AF6-120.1 antibody reognizes the I-A[b] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[k] and H-2[u] haplotypes. Reactivity with other haplotypes (e.g., d, f, g7, p, q, r, s) has not been observed.

550553 Rev. 3
フォーマットの詳細
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
550553 Rev.3
引用&参考文献
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開発者向け参考資料 (4)

  1. BD Biosciences Pharmingen. Unpublished results. .
  2. Hattori M, Buse JB, Jackson RA, et al. The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex. Science. 1986; 231(4739):733-735. (Biology). 参考文献を見る
  3. Stall AM. Personal Communication. .
  4. Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす
550553 Rev. 3

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.