BD Pharmingen™ Biotin Mouse Anti-Armenian and Syrian Hamster IgG1

For the sandwich hamster IgG (group 1) ELISA, biotin-conjugated mAb G94-56 is optimal for detection with purified anti-hamster IgG mAb HIG-632 (Cat. No. 550637) for capture and Cat. No. 553969 as protein standard. Biotin-conjugated G94-56 mAb may be used as a secondary reagent in immunofluorescent staining. For immunohistochemical staining, we recommend the use of biotinylated anti-hamster IgG mAb cocktail in our special formulation for immunohistochemistry, Cat. No. 550335.

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.