The RM2-5 monoclonal antibody specifically binds to the immunoglobulin superfamily adhesion molecule, CD2 (LFA-2). CD2 is a type I transmembrane glycoprotein that serves as the the major receptor for CD48 in the mouse. CD2 is involved in T-cell activation, immunoregulation, and thymocyte maturation. In the mouse, CD2 is expressed on peripheral T lymphocytes, B lymphocytes, and NK cells, and a subpopulation of intraepithelial T lymphocytes. CD2 is expressed throughout mouse thymic ontogeny, except for distinct subsets of the CD4-CD8- early thymocytes. In the mouse bone marrow, CD2 is expressed on B220+ sIg+ CD43- pre-B cells, but not on CD43+ pro-B cells. The RM2-5 antibody is one of a set of five anti-mouse CD2 monoclonal antibodies that were classified into two groups according to their mutual competition in binding to cell surface CD2 and which block CD2-mediated cell-cell adhesion.
The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40-nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.