Analysis of CD83 expression by mature dendritic cells (DC). C57BL/6 bone marrow-derived DC were cultured for 6 days with recombinant mouse GM-CSF (Cat. no. 554586), stimulated for 24 hours with lipopolysaccharide, then stained with FITC hamster anti-mouse CD11c mAb HL3 (Cat. no. 557400) and either APC Rat IgG1, κ isotype control mAb R3-34 (Cat. no. 554686, left panel) or APC mAb Michel-19 (right panel). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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Analysis of CD83 expression by mature dendritic cells (DC). C57BL/6 bone marrow-derived DC were cultured for 6 days with recombinant mouse GM-CSF (Cat. no. 554586), stimulated for 24 hours with lipopolysaccharide, then stained with FITC hamster anti-mouse CD11c mAb HL3 (Cat. no. 557400) and either APC Rat IgG1, κ isotype control mAb R3-34 (Cat. no. 554686, left panel) or APC mAb Michel-19 (right panel). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
製品詳細
BD Pharmingen™
Mouse (QC Testing)
Rat IgG1, κ
Mouse CD83 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
12522
AB_647164
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
APC Rat IgG1, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
554686
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FITC Hamster Anti-Mouse CD11c RUO
サイズ
0.1 mg
カタログ番号
557400
Recombinant Mouse GM-CSF RUO
サイズ
10 µg
カタログ番号
554586
558206 Rev. 1
抗体の詳細
Michel-19
The Michel-19 antibody reacts with CD83, a member of the immunoglobulin superfamily that is expressed on mature dendritic cells and activated T lymphocytes. Furthermore, thymic cortical epithelial cells express Cd83 transcripts. CD83 is involved in the regulation of T-cell development and immune responses, and its ligand is found on a subpopulation of splenic B lymphocytes.
558206 Rev. 1
フォーマットの詳細
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
558206 Rev.1
引用＆参考文献
Development References (5)
-
Berchtold S, Muhl-Zurbes P, Heufler C, Winklehner P, Schuler G, Steinkasserer A. Cloning, recombinant expression and biochemical characterization of the murine CD83 molecule which is specifically upregulated during dendritic cell maturation. FEBS Lett. 1999; 461:211-216. (Biology). View Reference
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Cramer SO, Trumpfheller C, Mehlhoop U, More S, Fleischer B, von Bonin A. Activation-induced expression of murine CD83 on T cells and identification of a specific CD83 ligand on murine B cells. Int Immunol. 2000; 12(9):1347-1351. (Biology). View Reference
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Fujimoto Y, Tu L, Miller AS, et al. CD83 expression influences CD4+ T cell development in the thymus. Cell. 2002; 108:755-767. (Biology). View Reference
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Wolenski M, Cramer SO, Ehrlich S, Steeg C, Fleischer B, von Bonin A. Enhanced activation of CD83-positive T cells. Scand J Immunol. 2003; 58(3):306-311. (Biology). View Reference
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Wolenski M, Cramer SO, Ehrlich S, et al. Expression of CD83 in the murine immune system. Med Microbiol Immunol (Berl). 2003; 192(4):189-192. (Biology). View Reference
558206 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
