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APC Rat Anti-Mouse CD209a
APC Rat Anti-Mouse CD209a

Flow cytometric analysis of CD209a expression on CD209a-transfected cells. Mouse CD209a-transfected CHO cells were stained with either APC Rat IgG2a, κ Isotype Control (Cat. No. 553932; dashed line histogram) or APC Rat Anti-Mouse CD209a antibody (Cat. No. 564928; solid line histogram).  The fluorescence histogram showing CD209a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of CD209a expression on CD209a-transfected cells. Mouse CD209a-transfected CHO cells were stained with either APC Rat IgG2a, κ Isotype Control (Cat. No. 553932; dashed line histogram) or APC Rat Anti-Mouse CD209a antibody (Cat. No. 564928; solid line histogram).  The fluorescence histogram showing CD209a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

製品詳細
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BD Pharmingen™
CD209; Cire; DC-SIGN; Dcsign; DC-SIGN1; CDSIGN
Mouse (QC Testing)
Rat IgG2a, κ
Peptide Derived from Extracellular Region of CD209a/CIRE
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739011
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564928 Rev. 1
抗体の詳細
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5H10

The 5H10 monoclonal antibody specifically binds to mouse CD209a, or CIRE, which is the most closely related homolog of human DC-SIGN. In the mouse, several homologs of human CD209 (DC-SIGN) have been described. CD209a is a 238-amino acid type-II transmembrane C-type lectin, with an extracellular carbohydrate recognition domain. It can bind to mannosylated ligands in a Ca++ dependent manner. CD209a is expressed by some conventional CD4+ and CD4-CD8- dendritic cells (DC) but not by CD8+ DC. It is also expressed on monocytes, splenic plasmacytoid pre-DC, macrophages, and resident peritoneal cells. CD209a expression is downregulated upon cellular activation. CD209a is thought to play a role in the internalization and presentation of antigens to initiate immune responses.

564928 Rev. 1
フォーマットの詳細
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC
Red 627-640 nm
651 nm
660 nm
564928 Rev.1
引用&参考文献
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Development References (7)

  1. Baribaud F, Pohlmann S, Sparwasser T, et al. Functional and antigenic characterization of human, rhesus macaque pigtailed macaque, and murine DC-SIGN. J Virol. 2001; 75(21):10281-10289. (Biology). View Reference
  2. Caminschi I, Corbett AJ, Zahra C, et al. Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int Immunol. 2006; 18(5):741-753. (Immunogen: Flow cytometry, Fluorescence activated cell sorting, Western blot). View Reference
  3. Caminschi I, Lucas KM, O'Keeffe MA, et al. Molecular cloning of a C-type lectin superfamily protein differentially expressed by CD8alpha(-) splenic dendritic cells. Mol Immunol. 2001; 38(5):365-373. (Biology). View Reference
  4. O'Keeffe M, Hochrein H, Vremec D, et al. Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8(+) dendritic cells only after microbial stimulus. J Exp Med. 2002; 196(10):1307-1319. (Clone-specific: Flow cytometry). View Reference
  5. Parent SA, Zhang T, Chrebet G, et al. Molecular characterization of the murine SIGNR1 gene encoding a C-type lectin homologous to human DC-SIGN and DC-SIGNR. Gene. 2002; 293(1):33. (Biology). View Reference
  6. Park CG, Takahara K, Umemoto E, et al. Five mouse homologues of the human dendritic cell C-type lectin, DC-SIGN. Int Immunol. 2001; 13(10):1283-1290. (Biology). View Reference
  7. Ponichtera HE, Shainheit MG, Liu BC, et al. CD209a expression on dendritic cells is critical for the development of pathogenic Th17 cell responses in murine schistosomiasis. J Immunol. 2014; 192(10):4655-4665. (Clone-specific: Flow cytometry). View Reference
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564928 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.