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APC Mouse Anti-Human CD32
APC Mouse Anti-Human CD32

Profile of peripheral blood lymphocytes analyzed by flow cytometry

APC Mouse Anti-Human CD32

Profile of peripheral blood monocytes analyzed by flow cytometry

APC Mouse Anti-Human CD32

Profile of peripheral blood granulocytes analyzed by flow cytometry

Profile of peripheral blood lymphocytes analyzed by flow cytometry

Profile of peripheral blood monocytes analyzed by flow cytometry

Profile of peripheral blood granulocytes analyzed by flow cytometry

製品詳細
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BD Pharmingen™
CD32a,FcγRIIa, FCGR2A; CD32b, FcγRIIb, FCGR2B; CD32c, FcγRIIc, FCGR2C
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Mouse BALB/c IgG2b, κ
K562 or FcγRII+ L cell lines
Flow cytometry (Routinely Tested)
20 µl
V MA128
AB_398665
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  7. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  8. An isotype control should be used at the same concentration as the antibody of interest.
559769 Rev. 7
抗体の詳細
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FLI8.26

The FLI8.26 monoclonal antibody specifically recognizes CD32 which is also known as Fcγ receptor type II (FcγRII). CD32 is a type I transmembrane glycoprotein that serves as a low affinity receptor for aggregated IgG. The FLI8.26 antibody recognizes a, b, and c forms of CD32 that are encoded by separate genes: CD32a/FcγRIIa (FCGR2A), CD32b/FcγRIIb (FCGR2B), and CD32c/FcγRIIc (FCGR2C). These forms are differentially expressed on monocytes, granulocytes, T cells, B cells, NK cells, or platelets. These receptors play various roles in mediating and regulating inflammation and immunity including phagocytosis, cytotoxicity, degranulation, or platelet activation.

559769 Rev. 7
フォーマットの詳細
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
559769 Rev.7
引用&参考文献
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Development References (10)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Båve U, Magnusson M, Eloranta ML, Perers A, Alm GV, Rönnblom L. Fc gamma RIIa is expressed on natural IFN-alpha-producing cells (plasmacytoid dendritic cells) and is required for the IFN-alpha production induced by apoptotic cells combined with lupus IgG.. J Immunol. 2003; 171(6):3296-302. (Clone-specific: Flow cytometry). View Reference
  3. Hogarth PM, Anania JC, Wines BD. The FcγR of humans and non-human primates and their interaction with IgG: implications for induction of inflammation, resistance to infection and the use of therapeutic monoclonal antibodies.. Curr Top Microbiol Immunol. 2014; 382:321-52. (Biology). View Reference
  4. Ierino FL, Hulett MD, McKenzie IF, Hogarth PM. Mapping epitopes of human Fc gamma RII (CDw32) with monoclonal antibodies and recombinant receptors. J Immunol. 1993; 150(5):1794-1803. (Biology). View Reference
  5. Lin G-X, Yang X, Hollemweguer E, et al. Cross-reactivity of CD antibodies in eight animal species. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:519-523.
  6. Meknache N, Jönsson F, Laurent J, Guinnepain MT, Daëron M. Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B).. J Immunol. 2009; 182(4):2542-50. (Clone-specific: Flow cytometry). View Reference
  7. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  8. Stuart SG, Simister NE, Clarkson SB, Kacinski BM, Shapiro M, Mellman I. Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium. EMBO J. 1989; 8(12):3657-3666. (Biology). View Reference
  9. Tomiyama Y, Kunicki TJ, Zipf TF, Ford SB, Aster RH. Response of human platelets to activating monoclonal antibodies: importance of Fc gamma RII (CD32) phenotype and level of expression. Blood. 1992; 80(9):2261-2268. (Biology). View Reference
  10. Van Den Herik Oudijk IE, Westerdaal NAC, De Haas M, et al. Binding heterogeneity within the CD32 panel of mAb. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:832-834.
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559769 Rev. 7

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.