Immunohistofluorescent analysis of CD31 expression by cells within human tonsil (Left Panel). A human tonsil cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 647 Mouse Anti-Human CD31 antibody (Cat. No. 558094, pseudo-colored green), Alexa Fluor® 488 Mouse Anti-Human CD4 antibody (Cat. No. 557695, pseudo-colored red), and BD Horizon™ BV421 Mouse Anti-Human CD19 antibody (Cat. No. 562440/562441, pseudo-colored blue). Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Analysis of CD31 in human peripheral blood granulocytes (Right Panel). Normal human peripheral blood cells were stained with either Alexa Fluor® 647 Mouse anti-Human CD31 (Cat. No. 558094, solid line) or Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (clone G155-178, Cat. No. 557715, dotted line). Flow cytometry was performed on a BD™ FACSCalibur flow cytometry system.
Immunohistofluorescent analysis of CD31 expression by cells within human tonsil (Left Panel). A human tonsil cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 647 Mouse Anti-Human CD31 antibody (Cat. No. 558094, pseudo-colored green), Alexa Fluor® 488 Mouse Anti-Human CD4 antibody (Cat. No. 557695, pseudo-colored red), and BD Horizon™ BV421 Mouse Anti-Human CD19 antibody (Cat. No. 562440/562441, pseudo-colored blue). Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Analysis of CD31 in human peripheral blood granulocytes (Right Panel). Normal human peripheral blood cells were stained with either Alexa Fluor® 647 Mouse anti-Human CD31 (Cat. No. 558094, solid line) or Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (clone G155-178, Cat. No. 557715, dotted line). Flow cytometry was performed on a BD™ FACSCalibur flow cytometry system.
製品詳細
BD Pharmingen™
PECAM-1
Human (QC Testing)
Mouse IgG2a
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
AB_397020
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558094 Rev. 2
抗体の詳細
M89D3
The M89D3 monoclonal antibody specifically recognizes CD31, which is also known as platelet endothelial cell adhesion molecule -1 (PECAM-1), a single chain, type I transmembrane protein of approximately 130-140 kDa. CD31 is a member of the immunoglobulin supergene family. Its extracellular region is composed of 574 amino acids and contains six C2-type Ig domains, and the intracellular region is composed of 118 residues. CD31 (PECAM-1) is expressed on endothelial cells, platelets, monocytes, neutrophils and NK cells. It has also been observed on subsets of T cells, but not on circulating B cells. It has been implicated in a number of cellular phenomena, including vascular wound healing, angiogenesis, and transendothelial migration of leukocytes and platelet aggregation in inflammatory responses.
558094 Rev. 2
フォーマットの詳細
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
558094 Rev.2
引用＆参考文献
Development References (6)
-
DeLisser HM, Newman PJ, Albelda SM. Platelet endothelial cell adhesion molecule (CD31). Curr Top Microbiol Immunol. 1993; 184:37-45. (Biology). View Reference
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Muller WA, Weigl SA, Deng X, Phillips DM. PECAM-1 is required for transendothelial migration of leukocytes. J Exp Med. 1993; 178(2):449-460. (Biology). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Simmons DL, Walker C, Power C, Pigott R. Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen. J Exp Med. 1990 June; 171(6):2147-2152. (Biology). View Reference
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Stockinger H, Gadd SJ, Eher R, et al. Molecular characterization and functional analysis of the leukocyte surface protein CD31. J Immunol. 1990 December; 145(11):3889-3897. (Biology). View Reference
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Vaporciyan AA, DeLisser HM, Yan HC, et al. Involvement of platelet-endothelial cell adhesion molecule-1 in neutrophil recruitment in vivo.. Science. 1993; 262(5139):1580-2. (Biology). View Reference
558094 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
