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Alexa Fluor® 647 Mouse Anti-Human CD194
Alexa Fluor® 647 Mouse Anti-Human CD194

Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes.  Human peripheral blood mononuclear cells (PBMC) were stained with the Alexa Fluor® 647 Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346 , left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557714).

Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes.  Human peripheral blood mononuclear cells (PBMC) were stained with the Alexa Fluor® 647 Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346 , left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557714).

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BD Pharmingen™
CCR4; C-C chemokine receptor type 4; CMKBR4; K5-5
Human (QC Testing)
Mouse C57BL/6 IgG1, κ
Human CCR4 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
HCDM 2006
1233
AB_396906
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
557863 Rev. 1
抗体の詳細
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1G1

The 1G1 monoclonal antibody specifically binds to CD194, also known as the human CC Chemokine Receptor type 4 (CCR4).  CCR4 is expressed on activated Th2 cells, regulatory T cells, activated NK cells, basophils, monocytes and platelets.  CCR4 is a seven-transmembrane, G-protein-coupled receptor, and is the specific receptor for CC chemokines, CCL22/MDC/Macrophage-Derived Chemokine and CCL17/TARC/Thymus and Activation-Regulated Chemokine. It has been reported that CCR4 mRNA is expressed mainly in the thymus and spleen. The human CCR4 gene has been mapped to chromosome 3p24. The purified form of this antibody has been reported not to be a neutralizing antibody.  The immunogen used to generate the 1G1 hybridoma has been reported to be human CCR4 transfected L1.2 mouse lymphoma cells.

557863 Rev. 1
フォーマットの詳細
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
557863 Rev.1
引用&参考文献
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Development References (4)

  1. Campbell JJ, Haraldsen G, Pan J, et al. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature. 1999; 400(6746):776-780. (Biology). View Reference
  2. D'Ambrosio D, Iellem A, Bonecchi R, et al. Selective up-regulation of chemokine receptors CCR4 and CCR8 upon activation of polarized human type 2 Th cells.. J Immunol. 1998; 161(10):5111-5. (Biology). View Reference
  3. Imai T, Chantry D, Raport CJ, et al. Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J Biol Chem. 1998; 273(3):1764-1768. (Biology). View Reference
  4. Power CA, Meyer A, Nemeth K, et al. Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. J Biol Chem. 1995; 270(33):19495-19500. (Biology). View Reference
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557863 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.