Flow cytometric analysis of somatostatin expression in human somatostatin-transfected 293F cells. Untransfected (dashed-line histogram) and human somatostatin-transfected (solid-line histogram) 293F cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and stained with Alexa Fluor® 488 Mouse Anti-Human Somatostatin (Cat. No. 566032).The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
Immunohistofluorescent staining of somatostatin in human islets of Langerhans. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), sections of formalin-fixed, paraffin-embedded human pancreas were blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Cat. No. SP-2001) as recommended by the manufacturer. LEFT PANEL: The section was stained with Alexa Fluor® 488 Mouse Anti-Human Somatostatin (Cat. No. 566032, pseudocolored red), and cell nuclei were counterstained with BD Pharmingen™ DAPI Solution (Cat. No. 564907, pseudo-colored green). RIGHT PANEL: The section was stained with Alexa Fluor® 488 Mouse Anti-Human Somatostatin (Cat. No. 566032, pseudocolored red), BD Horizon™ BV421 Mouse Anti-Glucagon (Cat. No. 565891, pseudocolored blue), and Alexa Fluor® 647 Mouse Anti-Insulin (Cat. No.565689, pseudocolored green). The photographs were performed on a standard epifluorescence microscope. Original magnification, 20x.
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Flow cytometric analysis of somatostatin expression in human somatostatin-transfected 293F cells. Untransfected (dashed-line histogram) and human somatostatin-transfected (solid-line histogram) 293F cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and stained with Alexa Fluor® 488 Mouse Anti-Human Somatostatin (Cat. No. 566032).The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
Immunohistofluorescent staining of somatostatin in human islets of Langerhans. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), sections of formalin-fixed, paraffin-embedded human pancreas were blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Cat. No. SP-2001) as recommended by the manufacturer. LEFT PANEL: The section was stained with Alexa Fluor® 488 Mouse Anti-Human Somatostatin (Cat. No. 566032, pseudocolored red), and cell nuclei were counterstained with BD Pharmingen™ DAPI Solution (Cat. No. 564907, pseudo-colored green). RIGHT PANEL: The section was stained with Alexa Fluor® 488 Mouse Anti-Human Somatostatin (Cat. No. 566032, pseudocolored red), BD Horizon™ BV421 Mouse Anti-Glucagon (Cat. No. 565891, pseudocolored blue), and Alexa Fluor® 647 Mouse Anti-Insulin (Cat. No.565689, pseudocolored green). The photographs were performed on a standard epifluorescence microscope. Original magnification, 20x.
製品詳細
BD Pharmingen™
growth hormone release-inhibiting factor, SST
Human (QC Testing), Mouse, Rat (Lack of Reactivity Confirmed in Development)
Mouse IgG2b, κ
Human Preprosomatostatin Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
AB_2739476
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Fixation Buffer RUO
サイズ
100 mL
カタログ番号
554655
Perm Buffer III RUO
サイズ
125 mL
カタログ番号
558050
Retrievagen A (pH 6.0) RUO
カタログ番号
550524
DAPI Solution RUO
サイズ
1 mg
カタログ番号
564907
566032 Rev. 1
抗体の詳細
U24-354
Somatostatin is produced by neuroendocrine neurons in the hypothalamus and δ cells in the islets of Langerhans of the pancreas. It is a regulatory hormone that affects neurotransmission, cell proliferation, and numerous hormones of the endocrine system. Via interaction with high affinity G protein-coupled somatostatin receptors, it inhibits the secretion of somatotropin (also known as growth hormone or GH), thyroid-stimulating hormone (thyrotropin or TSH), and most gastrointestinal and pancreatic hormones, including glucagon and insulin. The expression of somatostatin can be used to monitor the pancreatic differentiation of pluripotent stem cells.
The human somatostatin gene, SST, encodes the precursor molecule preprosomatostatin (amino acids 1-116), which is cleaved to form prosomatostatin (amino acids 15-116), which in turn is cleaved to form either of 2 alternative active somatostatin peptides, somatostatin-28 (amino acids 89-116) or somatostatin-14 (amino acids 103-116). The U16-850 monoclonal antibody detects human preprosomatostatin in somatostatin-producing cells.
566032 Rev. 1
フォーマットの詳細
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
566032 Rev.1
引用＆参考文献
Development References (4)
-
D'Amour KA, Bang AG, Eliazer S, et al . Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006; 24(12):1481-1483. (Biology). View Reference
-
Goodman RH, Montminy MR, Low MJ, Habener JF. Biosynthesis of rat preprosomatostatin.. Adv Exp Med Biol. 1985; 188:31-47. (Biology). View Reference
-
Kelly OG, Chan MY, Martinson LA, et al. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol. 2011; 29(8):750-756. (Biology). View Reference
-
Montminy MR, Goodman RH, Horovitch SJ, Habener JF. Primary structure of the gene encoding rat preprosomatostatin.. Proc Natl Acad Sci USA. 1984; 81(11):3337-40. (Biology). View Reference
566032 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
