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PE Perforin Reagent Set

PE Perforin Reagent Set

(RUO)
PE Perforin Reagent Set
Profile of permeabilized peripheral blood lymphocytes analyzed on a BD FACScan™ (BDIS, San Jose, CA). Cells were stained with anti-human perforin-PE (clone δG9; shaded histogram) or with an IgG2b-R-PE isotype control.
Profile of permeabilized peripheral blood lymphocytes analyzed on a BD FACScan™ (BDIS, San Jose, CA). Cells were stained with anti-human perforin-PE (clone δG9; shaded histogram) or with an IgG2b-R-PE isotype control.
製品詳細
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BD Pharmingen™
Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
RUO
AB_396419


説明

Perforin has a key role in cell-mediated cytotoxicity. It is a 70 kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases. NK cells are important for tumor surveillance and destruction and are involved in allograft rejections. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions.

Clone δG9 reacts with human and bovine perforin. It does not cross-react with mouse perforin. Purified granules from the human lymphoma cell line YT were used as immunogen. Clone δG9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections. The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.

調製と保管

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

製品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. An isotype control should be used at the same concentration as the antibody of interest.
556437 Rev. 12
構成品
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説明 数量/容量 Part Number EntrezGene ID
PE Mouse Anti-Human Perforin 100 Tests (1 ea) 51-65995X N/A
PE Mouse IgG2b, κ Isotype Control 100 Tests (1 ea) 51-66375X N/A
556437 Rev. 12
引用&参考文献
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開発者向け参考資料 (3)

  1. Hameed A, Fox WM, Kurman RJ, Hruban RH, Podack ER. Perforin expression in endometrium during the menstrual cycle. Int J Gynecol Pathol. 1995; 14(2):143-150. (Clone-specific: Flow cytometry). 参考文献を見る
  2. Hameed A, Olsen KJ, Cheng L, Fox WM 3rd, Hruban RH, Podack ER. Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992; 140(5):1025-1030. (Immunogen: Immunohistochemistry, Immunoprecipitation). 参考文献を見る
  3. Rukavina D, Balen-Marunic S, Rubesa G, Orlic P, Vujaklija K, Podack ER. Perforin expression in peripheral blood lymphocytes in rejecting and tolerant kidney transplant recipients. Transplantation. 1996; 61(2):285-291. (Clone-specific: Flow cytometry). 参考文献を見る
556437 Rev. 12

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.