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BD Stemflow™ PE Human Mesenchymal Stem Cell Lineage Antibody Cocktail, with Isotype Control
(RUO)Cells grown in BD Mosaic™ hMSC SF Cell Culture Environment (Cat. No. 355700) were detached using BD™ Accutase™ Cell Detachment Solution (Cat. No 561527) and then stained with the PE hMSC Negative Cocktail and a cocktail of antibodies containing FITC CD90, PerCP-Cy5.5 CD105 and APC CD73 (See cat. No.562245) (solid lines) or the corresponding isotype control cocktails (dashed lines). The plots were derived from gated events based on light scattering characteristics of the MSCs. Cells were analyzed using a BD™ LSRII flow cytometry system.
BD Pharmingen™ PE Human Mesenchymal Stem Cell Lineage Antibody Cocktail, with Isotype Control
PE Human Mesenchymal Stem Cell Lineage Antibody Cocktail, with Isotype Control
Regulatory Statusの凡例
Becton, Dickinson and Companyの書面による明示的な許諾を得た使用以外での製品の使用は固く禁じられています。
説明
Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues. MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes. MSCs also have immunomodulatory effects in vivo and in vitro. In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow. Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR. MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271. This lineage set includes the set of ISCT negative markers as a cocktail and also a corresponding isotype control cocktail.
Description of Set Components
Vial Contents Purpose
PE hMSC Negative Cocktail CD34 PE (Clone:581) Cocktail to identify potential contaminants
CD11b PE (Clone: ICRF44)
CD19 PE (Clone: HIB19)
CD45 PE (Clone: HI30)
HLA-DR PE (Clone: G46-6)
PE hMSC Isotype Control Negative Cocktail mIgG1, κ PE (Clone: X40) Corresponding isotype control for PE hMSC
mIgG2a, κ PE (Clone:G155-178) Negative Cocktail
調製と保管
製品通知
- Accutase is a registered trademark of Innovative Cell Technologies, Inc.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
コンパニオン製品
説明 | 数量/容量 | Part Number | EntrezGene ID |
---|---|---|---|
PE hMSC Negative Cocktail | 50 Tests (1 ea) | 51-9007661 | N/A |
PE hMSC Isotype Control Negative Cocktail | 50 Tests (1 ea) | 51-9007662 | N/A |
開発者向け参考資料 (5)
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Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Biology: Flow cytometry). 参考文献を見る
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Dominici M, Le Blanc K, Mueller I, et. al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-317. (Biology: Flow cytometry). 参考文献を見る
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Horwitz EM, Le Blanc K, Dominici M, et. al. Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy. 2005; 7(5):393-395. (Biology). 参考文献を見る
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Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-1301. (Biology: Flow cytometry). 参考文献を見る
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Sánchez L, Gutierrez-Aranda I, Ligero G, et al. Enrichment of Human ESC-Derived Multipotent Mesenchymal Stem Cells with Immunosuppressive and Anti-Inflammatory Properties Capable to Protect Against Experimental Inflammatory Bowel Disease. Stem Cells. 2010; 29(2):251-262. (Biology: Cell differentiation). 参考文献を見る
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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