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FITC Mouse Anti-p53 Set

FITC Mouse Anti-p53 Set

(RUO)
FITC Mouse Anti-p53 Set
Profile of permeabilized HT-29 colon adenocarcinoma cells analyzed on a FACScan™ (BDIS, San Jose, CA). Cells were stained with anti-p53 FITC (clone G59-12) or with a mouse IgG1 isotype control.
Profile of permeabilized HT-29 colon adenocarcinoma cells analyzed on a FACScan™ (BDIS, San Jose, CA). Cells were stained with anti-p53 FITC (clone G59-12) or with a mouse IgG1 isotype control.
製品詳細
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BD Pharmingen™
Human (QC Testing), Mouse, Rat (Tested in Development)
Intracellular staining (flow cytometry) (Routinely Tested)
RUO
AB_396556


説明

p53 is a 53 kDa nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpresssed in a variety of transformed cells and wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1b from adenovirus, and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle.

Clone G59-12 recognizes mutant and wild type human, mouse, and rat p53 tumor suppressor protein. Recombinant full-length human p53 was used as immunogen. The G59-12 clone was originally characterized by western blot analysis, immunoprecipitation and immunohistochemical staining. MOPC-21 antibody has unknown specificity. The G59-12 and MOPC-21 FITC conjugates are matched in F/P ratios determined experimentally by flow cytometric analysis.

調製と保管

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

推奨アッセイ手順

Positive control cell lines include SK-BR-3 human breast carcinoma cells (ATCC HTB-30) and A431 human epidermal carcinoma cells (ATCC CRL-1555). Jurkat human T (leukemia) cells (ATCC TIB-152) or MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as negative controls.

製品通知

  1. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
557026 Rev. 6
構成品
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説明 数量/容量 Part Number EntrezGene ID
FITC Mouse Anti-p53 100 Tests (1 ea) 51-14214X N/A
FITC Mouse IgG1, κ Isotype Control 100 Tests (1 ea) 51-13854X-7 N/A
557026 Rev. 6
引用&参考文献
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開発者向け参考資料 (4)

  1. Gjerset RA, Arya J, Volkman S, Haas M. Association of induction of a fully tumorigenic phenotype in murine radiation-induced T-lymphoma cells with loss of differentiation antigens, gain of CD44, and alterations in p53 protein levels. Mol Carcinog. 1992; 5(3):190-198. (Biology). 参考文献を見る
  2. Stein LS, Stoica G, Tilley R, Burghardt RC. Rat ovarian granulosa cell culture: a model system for the study of cell-cell communication during multistep transformation. Cancer Res. 1991; 51(2):696-706. (Biology). 参考文献を見る
  3. Vogelstein B. Cancer. A deadly inheritance. Nature. 1990; 348(6303):681-682. (Biology). 参考文献を見る
  4. Vojtesek B, Bartek J, Midgley CA, Lane DP. An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J Immunol Methods. 1992; 151(1-2):237-244. (Biology). 参考文献を見る
557026 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.