製品詳細
BD Pharmingen™
Anti-Keyhole Limpet Hemocyanin (KLH)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
Trinitrophenyl–Keyhole Limpet Hemocyanin
Flow cytometry, Isotype control (Routinely Tested)
0.5 mg/ml
AB_396575
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
推奨アッセイ手順
This immunoglobulin is useful as an isotype-matched negative control for immunofluorescent staining.
Product Notices
- An isotype control should be used at the same concentration as the antibody of interest.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557077 Rev. 3
抗体の詳細
B39-4
The B39-4 hybridoma was generated using cells from a rat that was immunized with the hapten-carrier conjugate, trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH). The B39-4 antibody is specific for KLH. It has an IgG2a, λ isotype and can serve as a immunoglobulin isotype control.
557077 Rev. 3
フォーマットの詳細
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
557077 Rev.3
引用＆参考文献
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557077 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.